Download Nyt ISO dokument

Nyt ISO dokument
Susceptibility testing of infectious agents
and evaluation of performance of
antimicrobial susceptibility test devices.
– Part 1: Reference method for testing the in
vitro activity of antimicrobial agents against
rapidly growing aerobic bacteria involved in
infectious diseases.
ISO reference metode
Mikrotitter bouillon fortynding
– Cation supplementeret Mueller Hinton
bouillon
– Inokulat: 5 x 105 CFU/ml (2-8 x 105 CFU/ml)
– Inkubations tp: 34-37C, atmosfærisk luft,
18 ± 2 timer.
Intermediate I
bacterial strain that is inhibited in vitro by a
concentration of an antimicrobial agent that is
associated with uncertain therapeutic effect
– NOTE: This class of susceptibility implies that an
infection due to the isolate may be appropriately
treated in body sites where the drugs are
physiologically concentrated or when a high dosage
of drug can be used.
– NOTE: This class also indicates a “buffer zone” that
should prevent small, uncontrolled, technical factors
from causing major discrepancies in interpretations.
ISO reference isolater
Staphylococcus aureus ATCC 29213
(NCTC 12973, CIP 103429, DSM 2569)
Enterococcus faecalis ATCC 29212
(NCTC 12697, CIP 103429, DSM 2570)
Escherichia coli ATCC 25922
(NCTC 12241, CIP 7624, DSM 1103)
Pseudomonas aeruginosa ATCC 27853
(NCTC 12973, CIP 54127, DSM 1117)
Streptococcus pneumoniae, ATCC 49619
(NCTC 12977)
Escherichia coli ATCC 35218 (DSM 5564)
ISO reference værdier
Identiske med kendte CLSI / Eucast værdier
Antibiotikum
S. aureus
ATCC 29213
E. faecalis
ATCC 29212
E. coli
ATCC 25922
P. aeruginosa
ATCC 27853
S. pneumoniae
ATCC 49619
Ampicillin
0,5–2
0,5–2
2–8
—
0,06–0,25
Cefaclor
1–4
—
1–4
—
1–4
Cefotaxim
1–4
—
0,03–0,12
8–32
0,03–0,12
Cefoxitin
1–4
—
2–8
—
—
0,25–1
1–4
—
—
0,06–0,5
0,06-0,25
1–4
0,5–2
2–8
0,03–0,12
1–4
2–8
Daptomycin**
Fusidin*
Norfloxacin