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Pit-and-Fissure Sealants - how sealants can provide a primary preventive means of reducing the need for operative treatment as 77% of the children 12 to 17 years old in the United States have dental caries in their permanent teeth.1 Introduction Fluorides are highly effective in reducing the number of carious lesions occurring on the smooth surfaces of enamel and cementum. Unfortunately, fluorides are not equally effective in protecting the occlusal pits and fissures, where the majority of carious lesions occur. Considering the fact that the occlusal surfaces constitute only 12% of the total number of tooth surfaces, it means that the pits and fissures are approximately eight times as vulnerable as the smooth surfaces. The placement of sealants is a highly effective means of preventing these diseses. A sealant is probably indicated if: • The fossa selected for sealant placement is well isolated from another fossa with a restoration. • The area selected is confined to a fully erupted fossa, even though the distal fossa is impossible to seal due to inadequate eruption. • An intact occlusal surface is present where the contralateral tooth surface is carious or restored; this is because teeth on opposite sides of the mouth are usually equally prone to caries. • An incipient lesion exists in the pit-and-fissure. • Sealant material can be flowed over a conservative class I composite or amalgam to improve the marginal integrity, and into the remaining pits and fissures to achieve a de facto extension for prevention. Other Considerations in Tooth Selection All teeth meeting the previous criteria should be sealed and resealed as needed. Where the cost-benefit is critical and priorities must be established, such as occurs in many public health programs, ages 3 and 4 years are the most important times for sealing the eligible deciduous teeth; . ages 6 to 7 years for the first permanent molars; and ages 11 to 13 years for the second permanent molars and premolars. Currently, 77% of the children 12-to-17years-old in the United States have dental caries in their permanent teeth. Many school days would be saved, and better dental health would be achieved in School Dental Health Clinic programs by combining sealant placement and rregular fluoride exposure Background of Sealants Buonocore first described the fundamental principles of placing sealants in the late 1960s.He describes a method to bond poly-methylmethacrylate (PMMA) to human enamel conditioned with phosphoric acid. Practical use of this concept however, was not realized until the development of bisphenol Aglycidyl methacrylate (Bis-GMA), urethane dimethacrylates (UDMA) and trithylene glycol dimethacrylates (TEGDMA) resins that possess better physical properties than PMMA. The first successful use of resin sealants was reported by Buonocore in the 1960s. VIDEO The Saliva Compartment The saliva is derived mainly from the major salivary glands the parotid, submandibular, and sublingual glands. Of these, the parotid elaborates a serous (watery, mucous-poor) fluid containing electrolytes, but is relatively low in organic substances. . The parotid gland secretes the majority of the sodium bicarbonate that is essential in neutralizing acids produced by cariogenic bacteria in the dental plaque, and the majority of the enzyme amylase that initiates intraoral digestion of carbohydrates. The submandibular gland secretes a mixed serous and mucous fluid, while the sublingual gland has a greater proportion of mucous output than the other major glands. The minor glands palatal, lingual, buccal, and labial salivary glands empty onto the mucus membrane in many locations on the palate, under the tongue, and on the inner side of the cheeks and lips. These minor glands are mainly mucous secreting glands that lubricate these surfaces and allows for improved mastication and passage of food substance into the esophagus. The minor salivary glands also contribute fluoride that bathes the teeth and enhances caries resistance. Pure saliva produced by the oral glands is sterile, until it is discharged into the mouth. When the fluids from all major and minor glands mix with each other, this secretion becomes known as whole saliva. Whole saliva is further altered by the presence of particles of food, tissue fluid, lysed bacteria, and sloughed epithelial cells. It becomes even more complex with the inclusions of living cells and their metabolic products, for example, bacteria and leucocytes, the latter derived from the gingival crevices and tonsils Functions of saliva The physical and chemical protective functions of saliva can be divided into five convenient categories (1)-lubrication, (2) flushing/rinsing, (3) chemical, (4) antimicrobial (includes antibacterial, antifungal and antiviral), and (5) maintenance of supersaturation of calcium and phosphate level bathing the enamel, helping to stymie demineralization and/or to aid remineralization of tooth structure. To reinforce the concept expressed in Peretz aptly opined that saliva can be considered similar to enamel but in a liquid phase. Lubrication and Flushing A very thin microscopic layer of mucus protects the oral hard and soft tissues from the often harsh and abrasive foods, as they are being chewed and swallowed. It also protects the soft tissues from dessication and the teeth from abrasion. The moistening of food by saliva facilitates chewing and swallowing. Speech is enhanced by the reduced friction between the dry tongue and soft tissues. Coversely, a lack of saliva (xerostomia) results in a greatly increased risk of caries with its accompaniment of an extremely annoying dry-mouth sensation. Chewing, swallowing and speaking can all be difficult and uncomfortable with dry-mouth syndrome and often requires frequent ameliorating sips of water. Flow Rate Providing a constant fluid flow is probably the most important defense function of the salivary glands, because it is the fluid that transports the buffering agents, the antimicrobials, and the mineral content of saliva to help control the equilibrium between the demineralization and remineralization of tooth structure. Also, the fluid output of the glands is essential for diluting acids, flushing food particles embedded around the teeth, clearing refined carbohydrates (acid-producing sugar substrates) and physically removing any displaced bacteria Oral fluids in contact with food particles results in solubilizing food substances that interact with the taste buds to provide an accurate assessment of taste.2