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Cloning of 16S rRNA genes amplified directly from normal and disturbed
Results ofsuggests
the culture
independent
characterization
vaginal microflora
a strong
association
between Atopobium
ofvaginae
the vaginal
by cloning
and microflora
bacterial vaginosis.
What is the role of L. iners?
Grade
Subject code
Age
Age
Species
(N
=
38)
Species
Number
of clones
Nombre
de clones
Lactobacillus crispatus [AF257097]
Lactobacillus gasseri [AF243144]
Lactobacillus jensenii [AF243159]
Lactobacillus vaginalis [AF243177]
Atopobium vaginae [AF325325]
Gardnerella vaginalis e [M58744]
Lactobacillus iners [Y16329]
Mobiluncus mulieris [AJ427625]
Peptostreptococcus anaerobius [L04168]
Peptoniphilus sp. [D14147]
Prevotella bivia 91% [L16475]
Prevotella buccalis 96.6% [L16476]
Sneathia (Leptotrichia) sanguinegens [L37789]
Uncultured Megasphaera sp. clone [AY271937]
Uncultured Actinobacteridae clone 86% [AB089070]
Unidentified clone 1 [AY207059]
I
W1
I
W2
51
124
66.1
18.5
I
W3
34
118
II
W4
38
107
99.1
II
W5
III
W6
III
W7
III
W8
49
69
41
72
46
125
28
169
1.4
0.0
84.1
41.7
36.0
0.0
80.5
4.1
12.4
3.0
44
70
99.2
0.9
0.8
1.4
0.0
7.2
1.6
Atopobium rimae [AF292371]
0.8
Verhelst nucleatum
et al. BMC
Microbiology6.52004
Fusobacterium
[AJ006964]
1.4
1.4
2.9
22.2
22.2
1.4
6.9
5.6
1.6
0.0
31.2
6.4
4.8
0.8
4.0
68.6
14.3