Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
MLAB 2434 – CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY Chapter 9 – Use of Colony Morphology for Presumptive ID of Microorganism Chapter – 9- Colony Morphology (cont’d) Initial Observation & Interpretation of Cultures “Read” routine cultures after 18 to 24 hours of incubation If cultures cannot be read after 24 hours, refrigerate to prevent overgrowth and drying Chapter – 9- Colony Morphology (cont’d) Compare growth on different media Examples: • If good growth on BAP but no growth on MAC, the bacteria is probably a gram positive organism • If MAC growth is pink, the organism is a lactose frementer Chapter – 9- Colony Morphology (cont’d) Gross Colony Characteristics Hemolysis on BAP (Chocolate agar does not show true hemolysis) • α hemolysis – green discoloration on the media Chapter – 9- Colony Morphology (cont’d) • β hemolysis – complete clearing of blood around colonies on BAP Chapter – 9- Colony Morphology (cont’d) • No hemolysis (sometimes called γ or gamma) Chapter – 9- Colony Morphology (cont’d) Size Margin or edge Elevation Density Color Consistency Pigment Odor Chapter – 9- Colony Morphology (cont’d) Growth of Organisms in Liquid Media Streamers or vines and puff balls are associated with certain streps Turbidity is produced by G- rods See pictures on page 321