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Culture Media II MBBS Dr Ekta Chourasia Lecturer, Microbiology Culture Media Indications/ Need for culture Isolate bacteria in pure cultures. Demonstrate their properties. Obtain sufficient growth for preparation of antigens & for other tests. Typing bacterial isolates. Antibiotic sensitivity. Estimate viable counts. Maintain stock cultures. Oct 07 Dr Ekta, Microbiology, GMCA Classification of Media Based on the consistency : Solid media Semisolid media Liquid media Oct 07 Dr Ekta, Microbiology, GMCA Classification Based on the function or property: - Simple media - Enriched media - Enrichment broth - Selective media - Indicator media - Differential media - Composite media - Transport media Oct 07 Dr Ekta, Microbiology, GMCA Constituents Of Media Agar or agar- agar Peptone - mixture of partially digested proteins Yeast or meat extract NaCl Melting point : 98°C Solidifying point : 42°C % of agar : Solid media (2%) Oct 07 Dr Ekta, Microbiology, GMCA Simple Media Also called Basal medium Most commonly used in routine labs. e.g. Nutrient broth, NA Oct 07 Dr Ekta, Microbiology, GMCA Enriched Media Blood, serum or egg added to the basal medium. To grow bacteria which are more exacting in nutritional requirements. e.g. BA, CA Oct 07 Dr Ekta, Microbiology, GMCA Enrichment Broth (media) For mixed cultures or materials containing more than one bacterium. Contains substances which stimulates wanted bacteria & inhibits unwanted bacteria. e.g. Tetrathionate broth Oct 07 Dr Ekta, Microbiology, GMCA Selective Media Substances added to a solid media to favour the growth of wanted bacteria. e.g. TCBS agar for Vibrio cholerae Oct 07 Dr Ekta, Microbiology, GMCA Indicator Media Change in the colour of indicator with the growth of bacteria. e.g. Sulphite in Wilson Blair media for Salmonella typhi. Oct 07 Dr Ekta, Microbiology, GMCA Differential Media To bring out differing characteristics of bacteria. e.g. MacConkey’s agar Oct 07 Dr Ekta, Microbiology, GMCA Composite Media For identification of isolates e.g. Triple Sugar Iron Transport Media For delicate organisms Delay in transit e.g. Stuart’s transport media Oct 07 Dr Ekta, Microbiology, GMCA