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Transcript 
pGLO
Bacterial Genetics
E. Coli structure
E. Coli structure
E. Coli structure
E. Coli structure
E. Coli structure
E. Coli structure
E. Coli structure
E. Coli structure
Plasmid
• Additional DNA found
in some bacteria
• Small loop
• Carries one or more
genes
• Can be transferred
from one bacteria to
another.
pGLO plasmid (original form)
ara B,
• ori- point of origin for
DNA replication
• araC- gene for
repressor protein in
arabinose operon
• araB, A and D- gene
ara A & ara D for arabinose
metabolism
pGLO plasmid (engineered form)
• Gene for arabinose
metabolism (ara BAD)
has been replaced with
GFP.
• Amipicillin resistance
has been added (bla)
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