Download Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates

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Transcript 
Enzymes that attack “N” substrates
 1. Urease
 2. Gelatinase
 3. Nitrate Reductase
Urease
Urea + water
Urea Broth
Urea
pH Indicator
Acidic = Yellow
Basic = Pink
ammonia + carbon dioxide
Gram
Reaction
Morphology
E. coli
(-)
Bacilli
Proteus
vulgaris
(-)
Salmonella
Shigella
O2
Ferment
Lactose
Urease
Facultative
(+)
(-)
Bacilli
Facultative
(-)
(+)
(-)
Bacilli
Facultative
(-)
(-)
(-)
Bacilli
Facultative
(-)
(-)
Urease Test
Gelatinase
Gelatin (solid)
Tryptic Soy Gelatin Agar
Gelatin (liquid)
Gelatinase Test
Nitrate Reductase
Nitrate
Nitrite + water
Facultative Bacteria
Nitrate Broth
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