Download Ex. 16 Oxygen Requirements

Oxygen Requirements
 1. Aerobes
 2. Anaerobes
 3. Facultative
 4. Microaerophilic
Agar Stabs & Agar Shakes
 1. Liquify 6 NA Deeps in boiling water
 2. Boil for 10 minutes to drive out dissolved oxygen
 3. Allow 3 NA Deeps to cool and solidify
 Agar Stabs
 4. Temper 3 NA Deeps in water bath at 55 degrees C
 Agar Shakes
Agar Stabs
 1. Single stab with Inoculating needle – straight
down, straight back up
 1. Micrococcus luteus
 2. Staphylococcus epidermidis
 3. Clostridium sporogenes


Clostridium rubrum
Clostridium butrycum
Agar Shakes
 1. Inoculate with Inoculating Loop - “shake” test tube
so bacteria are equally distributed
 1. Micrococcus luteus
 2. Staphylococcus epidermidis
 3. Clostridium sporogenes


Clostridium rubrum
Clostridium butrycum
Agar Stab
 Aerobic
Agar Stab
 Anaerobic
Agar Stab
 Facultative
Agar Stab
 Microaerophilic
Fluid Thioglycollate Broths (FTB)
 Sodium thioglycollate
 Binds to and removes free oxygen
 Resazurin
 Anaerobic indicator


O2 - PINK
No O2 - Clear
Oxygen Reqirements
Aerobic
Microaerophilic
Anaerobic