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Transcript 
WP2 BREEDING SYSTEMS
SEXUAL HYBRIDIZATION
SANITARY ASPECTS
GENETIC TRANSFORMATION
EMBRYOGENESIS
GENETIC CHARACTERISATION
G&H
WP2 BREEDING SYSTEMS
TWO STEP
ORGANOGENESIS
ONE STEP
ORGANOGENESIS
AXILLARY
BUDS
G&H
BASAL PLATES
WP2 BREEDING SYSTEMS
EMBRYOGENIC CELL SUSPENSIONS
P8
G&H
WP2 BREEDING SYSTEMS
Bombarded calli. Hygromycin 25 mg/l
G&H
WP2 BREEDING SYSTEMS
Control. Hygromycin 25 mg/l
G&H
WP2 BREEDING SYSTEMS
GUS activity. Selected calli
G&H
WP2 BREEDING SYSTEMS
G&H
Milestones & Deliverables
M&D 2000
Milestones:
Establishment of a micropropagated collection of garlic clones
Axenic material available for transformation experiments
done
done
Deliverables:
none planned
WP2 BREEDING SYSTEMS
G&H
Milestones & Deliverables
M&D 2001
Milestones:
Callus lines presenting high levels of transient expression of uidA gene
Highly transformable callus lines available for stable transformation experiments
Regeneration of transgenic plants steadily expressing reporter and selectable genes
Garlic transgenic plants available
done
done
in progress
in progress
Deliverables:
DP12 paper on reliable transformation of garlic
DP1 DP12c (added DP): paper on new system of garlic regeneration (I)
(2002)
DP12d (added DP): paper on new system of garlic regeneration (II)
delayed
done
done (2002)
WP2 BREEDING SYSTEMS
G&H
Milestones & Deliverables
M&D 2002
Milestones:
Regeneration of transgenic plants steadily expressing genes from the sulphur pathway
transgenic garlic plants with alterations in the sulphur metabolism available
delayed
delayed
Deliverables:
none
WP2 BREEDING SYSTEMS
G&H
DELIVERABLES
-Effect of light in the organogenic ability of garlic roots using a one step in vitro system
Martin N., Garrido J., Martín J. & Barandiaran X. (next month in the web)
-Effect of Flurprimidol in the organogenic ability of garlic roots using a one step in vitro system
Martin N., Garrido J., Martín J. & Barandiaran X. (next month in the web)
ADVANCES IN GARLIC TISSUE CULTURE AND TRANSFORMATION. DOCTORAL THESIS.
NIEVES MARTIN. NOVEMBER 14TH 2002.
WP2 BREEDING SYSTEMS
PEOPLE INVOLVED
XABIER BARANDIARAN
NIEVES MARTÍN
JOSÉ GARRIDO
JESÚS MARTÍN
G&H
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