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Intron and Exon in Eukaryotic Cells exon promotor 3’ exon intron exon intron DNA 5’ stop codon start codon Transcription 5’ 3’ mRNA Processing cap poly A tail Splicing Intron deleted Take place in nucleus mature mRNA To cytoplasm Juang RH (2004) BCbasics cDNA Is Reverse Transcribed from mRNA Baltimore, Dulbecco, Temin (1975) 5’ Reverse transcription 3’ mature mRNA RNA hydrolysis 3’ poly A tail TTTT 5’ 5’ DNA 3’ CCC 5’ GGG 3’ polymerase 5’ 3’ Juang RH (2004) BCbasics Two Libraries： cDNA Library vs Genomic Library Genes in expression Total Gene Chromosomal DNA mRNA Reverse transcription cDNA Vector: Plasmid Restriction digestion Complete gene Smaller Library Gene fragments Larger Library Vector: Plasmid or phage Juang RH (2004) BCbasics
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