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Download Creating a lipid biosynthesis hybrid: E. nemone
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Creating a lipid biosynthesis hybrid: E. nemone Teresa and Jodi Goals • Combine our research interests – Teresa: lipid biosynthesis – Jodi: coral symbiosis and genomics • Teach our students • Try something new • Keep it low risk Combine our research interests: Roles of Lipids in Coral Symbiosis and Bleaching Lipids involved in establishment of symbiosis Symbiotic unit Photosynthetic symbionts Anemone host Lipids produced during thermal stress and loss of symbiosis + E-nemone! Lipid Biosynthetic Pathways PE PC PA PS CDP-DAG PI PG-P PG E. coli pathway Eukaryotic pathway CL Eukaryotic only lipids Teach our students: A hybrid course to build E. nemone • Combine our two existing courses – Chem 145: Chemistry Research Techniques • for accelerated freshmen • 9 students – Biol 244: Genomics • sophomore/junior lab course • 16 students • Six week intersection Research questions: • Can we alter the lipidome of E. coli? • Can we learn about Anemone lipid biosynthesis and function? Week 1 Intro to project Context of project Brainstorming about genomics and biochemistry approaches Learn about E. coli and eukaryotic lipid biosynthesis pathways Week 2 Identify target genes in E. coli and anemone genomes Choose target enzymes Chem students search for E. coli genes Bio students search for genes in anemone genomes Week 3 Decide what is feasible to engineer and get started Presentation of genes/discussion of which genes to target Synthetic Bio (Bio Bricks) discussion Design primers Week 4 Build the hybrid E. nemone: PCR, ligation, transformations, colony PCR Week 5 Characterize lipids: Grow cells, extract lipids, TLC, MS Week 6 Presentations Discussion: what would we do next? Synthetic Biology Approach We can use parts from the mini-registry pLac --- RBS --- RFP:GOI --- tt pLac --- RBS --- GOI --- RFP --- tt pLac --- RBS --- GOI --- tt GOI = Gene of Interest RFP = Red Fluorescent Protein Outcomes • Chem students learn genomics • Genomics students learn biochemistry • Development of interdisciplinary collaborations • Introduce Synthetic Biology to Vassar • Develop tools to enhance scholarship • Engage students in REAL research!
