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The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD).
Furthermore, it has a proven activator domain (A) and repressor domain (R).
Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote activation of target genes.
Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit activation of target genes.
30pts
pA site
Arl
E1
E2
E4
R
HD
A
SD
SD SA
Do not express TxFactors E1, E2, E3 or E4
Express TxFactors E1
Express TxFactor E1, E2,
Express TxFactors E1, E2, E3
Express TxFactors E1, E2, E3, E4
E3
means enhancer binding site
30 cells in
WT Antennal
Tissue
SA
RSS sites
LoxP site
RBPa binding site
(where RBP would bind in the transcribed RNA)
Remember that CreERT2 functions
Only when Tamoxifen is present
-Arl expression is normally dependent on all four TxFactors (E1, E2, E3, and E4) being present.
However, if E4 is absent in the DNA, then Arl can also be expressed in cells with both E1, E2 or with E1, E2 and E3.
-Rag 1 is expressed only in cells expressing both TxFactors E1 and E2.
-Rag 2 is expressed only in cells expressing all three TxFactors: E1, E2 and E3.
-A transgene exist in the genome for CreERT2 , but can be only activated by cells expressing AHD protein.
-RNA binding protein alpha inhibits splicing at HD.
-RNAa is only expressed in cells expressing both E1, E2.
All questions are 5pts each
In which cells will DNA rearangement
occur?
EXAM version A
In which cells will CreERT2 be expressed?
Quiz 11: Biol 203 Fall 2013
Name:_______________
Instructor Name
_______________
In which cells will DNA be deleted in the
presence of Tamoxifen (addition of
Tamoxifen is only after cells have formed)?
In which cells will unspliced mRNA be made
for Arl?
In which cells is Arl expressed prior to RSS
mediated recombination?
In which cells can Cre mediated
recombination and RSS recombination
BOTH occur in the genome of a cell?