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TORTORA • FUNKE
• CASE
Microbiology
AN INTRODUCTION
EIGHTH EDITION
B.E Pruitt & Jane J. Stein
Chapter 9, part B
Biotechnology and Recombinant DNA
PowerPoint® Lecture Slide Presentation prepared by Christine L. Case
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Genetic Engineering
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.11.1
Genetic Engineering
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.11.2
Making a Gene Product
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.12.1
Making a Gene Product
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.12.2
E. coli
• Used because it is easily grown and its genomics are
known
• Need to eliminate endotoxin from products
• Cells must be lysed to get product
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Making a Gene Product
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.13
Therapeutic Applications
• Subunit vaccines
• Nonpathogenic viruses carrying genes for pathogen's
antigens as vaccines
• Gene therapy to replace defective or missing genes
• Human Genome Project
• Nucleotides have been sequenced
• Human Proteome Project may provide diagnostics
and treatments
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Random Shotgun Sequencing
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.14
Scientific Applications
• Understanding of
DNA
• Sequencing
organisms'
genomes
• DNA fingerprinting
for identification
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.16
Southern Blotting
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.15.1
Southern Blotting
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.15.2
Southern Blotting
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.15.3
Agricultural Applications
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Table 9.2
Genetic Engineering Using Agrobacterium
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Figure 9.18
Safety Issues and Ethics
• Avoid accidental release
• Genetically modified crops must be safe for
consumption and for the environment
• Who will have access to an individual's genetic
information?
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings