Download Molecular genetics - AIS3

► Research Institute from University of Belgrade
► Affiliated center of ICGEB, Trieste, Italy
► Research and Advanced Education in Molecular Biology
► Services
► Promotion of Science
A brief history….
At the Conference in Belgrade, July 1982, organized by
Yugoslav Federal Government and UNIDO,
representatives of sixty countries decided to establish
International Centre for Genetic Engineering and
Biotechnology (ICGEB), an international organization,
consisting of one Center and a network of Affiliated
National Centers.
On February, 26 1986 SFRY Parliament
proclaimed the Law of Ratification of
the Statute of ICGEB and the
Institute of Molecular Genetics and Genetic Engineering
(IMGGE) was established as Affiliated Centre.
IMGGE: research focal points
FUNDAMENTAL RESEARCH
molecular biology
molecular genetics
recombinant DNA technology
biotechnology
Institute's projects are mostly focused on research related to
analysis of genome organisation and regulation of gene
expression in various organisms
APPLICATION
76 Researchers
8 Administration and Support Units Staff
7 Principal Research Fellows
7 Senior Research Associates
1 Scientific advisor
29 Research Associates
32 Research Assistants
Research Trainees
 Experimental and theoretical education
Completed theses and dissertations
B.Sc.= 168 Theses: M.Sc.= 87
Ph.D.= 52
Total: over 400
h-index: 31
IMGGE projects:
Ministry of Education and Science, R. Serbia
ICGEB
UNIDO
European projects (FP6, FP7, SEE-ERA-NET, REGPOT)
Department of Energy (DOE), USA
National Institute of Health (NIH), USA
Wellcome Trust, UK
Billateral cooperation
(Bulgaria, France, the Netherlands, Czech Republic
Spain, Portugal, Slovenia)
Laboratory for molecular hematology
Laboratory for molecular biology
Laboratory for human molecular genetics
Laboratory for microbial molecular genetics and ecology
Laboratory for molecular genetics of industrial microorganisms
Laboratory for plant molecular biology
 Science outreach activities
 Researcher’s Nights
 Science Fair
 Open Day
 Interactions with schools
 Elementary and High School visits
Little School of DNAlogy
Laboratory for molecular hematology
Head: Sonja PAVLOVIĆ, PhD (e-mail: [email protected])
Associates:
Maja STOJILJKOVIĆ PETROVIĆ, PhD
Gordana NIKČEVIĆ, PhD
Branka ZUKIĆ, PhD
Nataša TOŠIĆ, PhD
Tatjana KOSTIĆ, PhD
Teodora KARAN-ĐURAŠEVIĆ, BSc
Vesna SPASOVSKI, BSc
Milena RADMILOVIĆ, BSc
Biljana STANKOVIĆ, BSc
Nikola KOTUR, BSc
Kristel KLAASSEN, BSc
Sanja SRZENTIĆ, BSc
Laboratory for molecular hematology
Research Focus
MOLECULAR GENETICS of leukemia, thalassemia syndromes,
inflammatory bowel diseases, phenylketonuria
PHARMACOGENETICS - novel pharmacogenomic markers
Genetic diagnosis and molecular monitoring in the management of leukemia
Knowledge acquired through the fundamental research has been applied in
diagnostic and therapeutic protocols in Serbia and region
Laboratory for molecular hematology
Ongoing Projects
Integrative Interdisciplinary Research Project:
•Rare diseases: Molecular Pathophysiology, Diagnostic and Therapeutic
Modalities and Social, Ethical and Legal Aspects (MESRS, 2011-2014)
Collaborative International Projects:
•European LeukemiaNet - “Strengthen and develop scientific and technological excellence
in research and therapy of leukemia (CML, AML, ALL, CLL, MDS, CMPD) by integration
of the leading national leukemia networks and their interdisciplinary partner groups in Europe”
(EU, FP6-LSHC-CT-2004, 2004-2011)
• ENERCA 3 – “Framework of Community Action Programme on Rare Diseases” (2009-11)
Projects for Promotion of Science:
• Researchers’Night Fever „RENIFEVER“
(EU, CSA-SA, FP7-PEOPLE-2011-NIGHT- 287432, 2011)
• A short course of DNAlogy (MNTRS, 2011)
Laboratory for molecular hematology
Ongoing Projects
Integrative Interdisciplinary Research Project:
•Rare diseases: Molecular Pathophysiology, Diagnostic and Therapeutic
Modalities and Social, Ethical and Legal Aspects (MESRS, 2011-2014)
Collaborative International Projects:
•European LeukemiaNet - “Strengthen and develop scientific and technological excellence
in research and therapy of leukemia (CML, AML, ALL, CLL, MDS, CMPD) by integration
of the leading national leukemia networks and their interdisciplinary partner groups in Europe”
(EU, FP6-LSHC-CT-2004, 2004-2011)
• ENERCA 3 – “Framework of Community Action Programme on Rare Diseases” (2009-11)
Projects for Promotion of Science:
• Researchers’Night Fever „RENIFEVER“
(EU, CSA-SA, FP7-PEOPLE-2011-NIGHT- 287432, 2011)
• A short course of DNAlogy (MNTRS, 2011)
Laboratory for molecular hematology
Application
Molecular diagnostics
• Monogenic diseases (thalassemia syndromes, phenylketonuria)
• Hematological malignancies (ALL, AML, CML, CLL, lymphomas)
• Inflammatory diseases (Crohn’s disease, Perthes disease and rheumatoid
arthritis)
Pharmacogenomics
• Mercaptopurine drug therapy (TPMT )
• Irinotecan drug therapy (UGT1A1)
Laboratory for molecular biology
Head: Dragica RADOJKOVIĆ, PhD
Professor Emeritus: Professor Ana SAVIĆ, PhD
Snežana KOJIĆ, PhD
Jelena KUŠIĆ, PhD
Aleksandra DIVAC, PhD
Djordje FRANCUSKI, PhD
Aleksandra NIKOLIĆ, PhD
Valentina ĐORĐEVIĆ, PhD
Ljiljana RAKIĆEVIĆ, PhD
Marija STANKOVIĆ, PhD
Branko TOMIĆ, BSc
Mila LJUJIĆ, BSc
Aleksandra NESTOROVIĆ, BSc
Iva PRUNER, BSc
Laboratory for molecular biology
Research Focus
- Genotype–phenotype correlation
-Identification of mutations and polymorphisms in CFTR gene in CFTR
related disease (first data on type and frequency of CFTR mutations in Serbian
population - CF Network)
Application of molecular biology in medicine
- Study of skeletal muscle specific genes and DNA replication in eukaryotes
(DNA-protein interactions during the initiation of DNA replication)
Representatives of the Republic of Serbia in ORPHANET (The portal of rare
diseases and orphan drugs) and EPMA (The European Association for
Predictive, Preventive & Personalised Medicine)
Laboratory for molecular biology
Ongoing Projects
Complex diseases as a model system for phenotype modulation structural and functional analysis of molecular biomarkers (funded
by MESRS, 2011-2014)
Structural alterations of DNA induced by initiator protein (funded by
ICGEB, 2009-2011)
Evaluation of muscle specific protein Ankrd2/Аrpp in different
myopathies and its potential use as a diagnostic marker (funded by
MESRS/DAAD, 2011-2012)
EU project “EuroGentest2“, FP7-261469 (2011-2013)
Laboratory for molecular biology
Application
MOLECULAR DIAGNOSTICS
Cystic fibrosis
Alpha1 antitrypsin deficiency
Thrombophilia
Lactose intolerance
FV Leiden
PAI-1 4g/5g
FII G20210A
MTHFR C677T
PHARMACOGENETICS
tailoring anticoagulant therapy (VKORC1, CYP2C9)
tailoring antiagregation therapy (CYP2C19)
NONINVASIVE MOLECULAR DIAGNOSTICS OF SOLID TUMORS
Laboratory for human molecular genetics
Head: Milena STEVANOVIĆ, PhD
Corresponding member of Serbian Academy of Sciences and Arts
Associates:
• Marija MOJSIN, PhD
• Nataša KOVAČEVIĆ-GRUJIČIĆ, PhD
• Jelena POPOVIĆ, PhD
• Andrijana KLAJN, PhD
• Danijela DRAKULIĆ, MSc
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Isidora PETROVIĆ, MSc
Milena MILIVOJEVIĆ, BSc
Jovana JASNIĆ, BSc
Dijana TAKIĆ, BSc
Slobodan DAVIDOVIĆ, BSc
Laboratory for human molecular genetics
Research Focus
- Studying the regulation of expression and function of the human SOX genes
(cloning and characterization of new human SOX genes - SOX14, SOX18 and
SOX20)
- Application of fluorescent in situ hybridization (FISH) for detection of
numerical and structural chromosome aberrations in humans
- Devlopement of methods for genotyping of κ-casein and β-lactoglobulin and
for the selection of desirable variants that have an effect on milk production traits
in cattle
- Ancient DNA studies Molecular genetic analysis of the populations residing in
the Southwestern region of the Serbia from Bronze Age up to Middle Ages
Laboratory for human molecular genetics
Ongoing Projects
Studying signal transduction pathways and epigenetic mechanisms that
control human SOX genes expression: further insight into their roles
in cell fate determination and differentiation (project No.: 173051).
Ministry of Science and Technological Development, Republic Serbia,
2011-2014.
Ethnogenesis of the Serbs during the Middle Ages (until c.1250): a
comparative analysis of the historic-cultural heritage, genetic material and
artifacts of material culture from the aspect of analytical chemistry
(project No.: 47025). Subproject: Molecular genetic analysis of the
populations residing in the Southwestern region of the Serbia from Bronze
Age up to Middle Ages. Ministry of Science and Technological
Development, Republic Serbia, 2011-2014.
Studying the molecular mechanisms involved in maintaining pluripotency
and differentiation of stem cells (No.: Ф-24). Serbian Academy of
Sciences and Arts, 2010-.
Regulation of human SOX18 gene expression and its role in angiogenesis
(project No.: CRP/YUG07-01). The International Centre for Genetic
Engineering and Biotechnology (ICGEB), Italy, 2008-2010.
Laboratory for human molecular genetics
Application
FISH (Fluorescent in situ hybridization ) based diagnostics
for the detection:
Microdeletion syndromes (DiGeorge and Prader-Willi / Angelman)
Rare mosaic karyotypes
Complex structural chromosomal rearrangements.
Marker assisted selection for the economic trait loci in cattle
Genotyping of κ-casein and β-lactoglobulin and selection of desirable variants
that have an effect on milk production traits in cattle.
Мolecular diagnostics of BLAD (Bovine Leukocyte Adhesion
Deficiency) and porcine stress syndrome.
Laboratory for microbial genetics
and ecology
Head: Branka VASILJEVIĆ, PhD (e-mail: [email protected])
Associates:
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Ivana MORIĆ, PhD
Jasmina NIKODINOVIĆ RUNTIĆ, PhD
Lidija ŠENEROVIĆ, PhD
Lidija ĐOKIĆ, PhD
Tatjana ILIĆ TOMIĆ, PhD
Sandra VOJNOVIĆ, PhD
Tanja Narančić, PhD
Nada STANKO
Sanja BAJKIĆ, BSc
Laboratory for microbial genetics
and ecology
Research Focus
- former Laboratory for Molecular Genetics of Actinomycetes
MICROBIAL DIVERSITY ASSESSMENT
Metagenomic assessment of bacterial communities (cultural heritage, air, water,
sediment samples)
GREEN BIOTECHNOLOGY
Bioremediations (aromatic hydrocarbons, heavy metals)
Waste conversion to value-added materials
Biocatalysis
PRODUCTION OF BIOACTIVE COMPOUNDS
Isolation and screening for novel bioactive compounds (such as antibiotics, antitumor
drugs, immunosuppressants etc.)
Laboratory for microbial genetics
and ecology
Ongoing Projects
• Search for new bioactive secondary metabolites
(Bilateral cooperation, Countries: Serbia, Czech Republic,
2006-)
• Microbial diversity in the copper mine Bor
(ICGEB/CRP/YUG09-03, 2010-2012)
• Microbial diversity study and characterization of beneficial
environmental microorganisms
(funded by MES, 173048, 2011-2014)
Laboratory for microbial genetics
and ecology
Application
Bioremediation
Microbial degradation of different pollutants such as
phenol, benzene, toluene, ethylbenzene, xylenes, styrene etc.
Potential pharmaceutical application
Production of secondary metabolites such antibiotics,
antitumor drugs, immunosuppressants etc.
Laboratory for molecular genetics of
industrial microorganisms
Head: Milan KOJIC, PhD
Associates:
Ljubisa TOPISIROVIC, PhD
Djordje FIRA, PhD
Jelena LOZO, PhD
Jelena BEGOVIC, PhD
Sanja MIHAJLOVIC, Ph.D
Katarina VELJOVIC, PhD
Jovanka LUKIC, BSci
Gordana UZELAC, BSc
Ivana STRAHINIC, PhD
Natasa GOLIC, PhD
Amarela TERZIC-VIDOJEVIC, PhD
Branko JOVCIC, PhD
Maja TOLINACKI, PhD
Milica NIKOLIC, PhD
Goran VUKOTIĆ, BSc
Laboratory for molecular genetics of
industrial microorganisms
Research Focus
COLLECTION OF AUTOCHTONOUS LACTIC ACID BACTERIA (LAB) from
different homemade, artisanal fermented products traditionally manufactured in
households at very specific ecologically isolated regions throughout Western Balkan
region
Molecular characterization of LAB (genome organization, proteinase production,
aggregation factors, exopolysaccharide characterization, in vitro an in vivo probiotic
properties)
Construction of vectors for genetic manipulation of LAB that facilitate elucidation of
gene cloning and investigation of their expression and regulation
Molecular characterization of natural isolates of Bacillus sp. and Pseudomonas sp.
Laboratory for molecular genetics of
industrial microorganisms
Ongoing Projects
- Genes and molecular mechanisms promoting probiotic activity of LAB from Western Balkan
- Molecular characterization of bacteria from the genera Bacillus and Pseudomonas, as potential
agents for biological control
- Development of new incapsulation and enzyme technology for the production of biocatalysts
and bioactive food components to increase its competitiveness, quality and safety
funded by MESRS
- In vitro and ex vivo interaction of probiotic strains, producers of exopolysaccharides, with
intestinal epithelial cells (GUT-EPS)
funded by Serbian-Spain Bilateral Scientific Collaboration
- Study of proteolitic activity of lactic acid bacteria
funded by Serbian-France Bilateral Scientific Collaboration “Pavle Savic”
- Characterisation of spectinomycin induced chromosomal inversion in Lactococcus lactis subsp.
lactis bv. diacetylactis S50
funded by ICGEB-CRP Project, Italy
- Conservation and standardisation of traditional technologies of fermented milk products based on
autochthonous lactic acid bacteria
funded by SEE-ERA.NET (under FP7)
Application
Collection of autochtonous lactic acid bacteria
I - from traditionaly manufactured
fermented food products
II - of human
origin
Isolation and
Bacteriocin
production
characterizati
on
DNA fingerprinting
DNA
sequencing
Proteinase Production of
production aggregation
factors
LMGIM bacterial culture collection serves as a source of bacteria for:
Application of different probiotic
cultures
in food and pharmaceutical
industry
Laboratory for plant molecular biology
Head: Vesna MAKSIMOVIĆ, PhD
Associates:
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Svetlana Radović, PhD
Jovanka Miljuš Đukić, PhD
Jelena Samardžić, PhD
Gordana Timotijević, PhD
Dragana NIKOLIĆ
Jelena ALEKSIĆ ,PhD
Bojana BANOVIĆ, BSC
Živko JOVANOVIĆ, BSc
Nemanja STNISAVLJEVIĆ, BSc
Dragana NIKOLIĆ, BSc
Laboratory for plant molecular biology
Research Focus
GENETIC ENGINEERING TECHNIQUES IN CROP IMPROVEMENT:
Plant diversity as a rich source of genetic diversity – new genes for
improvement of
economically important crop species (tolerance to drought, cold, nutrient
deficiency and metal stress)
- Structure and expression of buckwheat genes related to stress response:
a) known function (seed storage proteins) b) undefined function (possibly involved in
protein degradation and processing - apsartic proteinase and metallothionenin)
c) transcription factors (DREB1 and DREB2)
- expression profiling of conserved micro RNAs (miR398 and miR408), important
regulatory molecules influencing plant stress responses (Fabaceae and Poaceae)
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Molecular analysis of self-incompatibility (SI) in Prunus sp. (gametophytSI) and
buckweat (dimorphic SI)
Research on genetic diversity of wild plant species and landraces (genealogical
and evolutionary relations)
Laboratory for plant molecular biology
Ongoing Projects
Basic research project funded by MESRS (2011-2014)
“Molecular mechanisms of plant response to abiotic stress and genetic
biodiversity of plant species important for agriculture and biotechnology”
Bilateral collaboration : France, Portugal, Slovenia
Approaches : Molecular genetics
THE ROLE OF TRANSCRIPTION FACTORS AND SMALL RNAs IN ABIOTIC STRESS
RESPONSE IN PLANTS
Molecular ecology
GENETIC DIVERSITY OF PLANT SPECIES
Balkan region as hot spot of biodiversity- wild populations and landraces
SEE ERA NET Plus, Joint European Research Project, 2011-2012
“Sustainable preservation of indigenous South East European legumes and
their traditional food and feed products-SEE LEGUMES”
Laboratory for plant molecular biology
Application
BIOTECHNOLOGICAL APPLICATION OF BUCKWHEAT
GENES/PROMOTERS: Storage proteins, Metallothioneins, Aspartic proteinases
GM plant with high nutritional quality proteins;
bioremediation
biosensors
APPLICATION OF GENETIC ENGINEERING TECHNIQUES
IN AGRICULTURE:
Plant diversity as a rich source of genetic diversity – new genes for improvement of
economically important crop species (tolerance to drought, cold, metal stress).
Collaborations with agricultural institutes:
Institute of Field and Vegetable Crops, Novi Sad
Fruit Research Institute - Čačak
Institute for Vegetable Crops, Smederevska Palanka
Institute for Forage Crops, Kruševac
SERVICE
GMO TESTING (Authorized by Ministry of Agriculture, Trade,
Forestry and Water Managementt, Republic of Serbia)
COLLABORATION WITH INSTITUTES
FROM ITALY
University of Padova, CRIBI Genomics Group
Universit degli Studi di Milano, Dipartimento di Scienze Biomolecolari
e Biotecnologie
Istituto San Raffaele, Dipartimento di Neuroscienze, Unit di Ricerca in
Neuroscienze Molecolari e Cellulari. Milano
ICGEB, Trieste
Centro Ricerca M. Tettamanti, Clinica Pediatrica Universita di MilanoBiccoca, H. San Gerardo, Monza
University of Cagliari, Dipartimento di Scienze Biomediche e Biotecnologie,
Cagliari, Sardinia,
Universit di Roma La Sapienza, Dpt. Biologia e Biotecnologie
'Charles Darwin'
European Institute of Oncology, Milano, Proteomics and Functional
Genomics, Dept. of Experimental
Oncology
University of Turin-DIVAPRA
THANK YOU
www.imgge.bg.ac.rs