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ResistVir
Mechanisms and sources of resistance
Josef SPAK
Institute of Plant Molecular Biology
Academy of Sciences of the Czech Republic, Ceske Budejovice
[email protected] http://www.umbr.cas.cz
Experience and Interests
• Brassica viruses - TuMV, CaMV, TYMV, RaMV
• Fruit ilarviruses - ApMV, PDV, PNRSV
• Small fruit viruses
– Strawberry - Nepoviruses, Aphid transmissible v.
– Raspberry - Nepoviruses, RBDV
– Currants - BRV, phytoplasma
Brassica viruses
•
TuMV - EU Project „Disease management of turnip mosaic virus
in oilseed rape“. Coordinator J.A. Walsh HRI, Wellesbourne, U.K.
–
–
Lehmann P, Petrzik K, Jenner C, Greenland A, Špak J, Kozubek E, Walsh JA.: Nucleotide and
amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible
involvement in the interaction with the brassica resistance gene TuRB01. Physiological and
Molecular Plant Pathology 51: 195 - 208, 1997.
Tomimura K, Špak J, Katis N, Jenner CE, Walsh JA, Gibbs AJ, Ohshima K. Comparisons of the
genetic structure of populations of Turnip mosaic virus in west and east Eurasia. Virology 330,
408-423, 2004.
•
TYMV - seed transmission, field resistence in oilseed rape
•
RaMV - variability of world isolates
–
Špak J, Kubelková D.: Serological variability among European isolates of radish mosaic virus.
Plant Pathology 49: 295 - 301, 2000.
–
current work - full sequence, genome variability
CP variability of ilarviruses
Distribution of mutations on the CP genes
ApMV
ApMV
PDV
PNRSV
203/675
104/590
153/660
214/682
Host-virus relationships
Phylogeny of PNRSV
Plum isolates
Cherry isolates
No correlation between host plant, CP sequence, geographical origin
Fruit ilarviruses - ApMV, PDV, PNRSV
• Petrzik K. (2005) Capsid protein sequence gene analysis of
Apple mosaic virus infecting pears Eur. J. Plant Pathol. 111 355360
• Petrzik K., O.Lenz (2002) Remarkable variability of Apple
mosaic virus capsid protein gene after the nucleotide position
141 Archives of Virology 147 1275-1285
• Vašková D., K.Petrzik, J.Špak (2000) Molecular variability of the
capsid protein of the prune dwarf virus Eur. J. Plant Pathol. 106
573-580
• Vašková D., K.Petrzik, R.Karešová (2000) Variability and
molecular typing of the woody-tree infecting necrotic ringspot
ilarvirus Archives of Virology 145 699-709
Small fruit viruses
• Strawberry
– Nepoviruses
– SVBV – full sequence, genome variability
– SVBV, SMYEV, SCV, SMoV
 5th Framework project „Virus Detector“, coordinated by
Wageningen UR, Netherlands
• Raspberry
– Nepoviruses
– RBDV
• Ribes – current work
– Black currant reversion disease
– Full blossom disease
Small fruit viruses
•
THOMPSON, J.R., WETZEL, S., KLERKS, M.M., VASKOVÁ, D., SCHOEN,
C.D., JELKMANN, W., SPAK, J.: Multiplex RT-PCR detection of four aphidborne strawberry viruses in Fragaria spp. in combination with a plant mRNA
specific internal control. - Journal of Virological Methods 111: 85-93, 2003.
•
KLERKS MM, LINDNER JL, VASKOVA D, SPAK J, THOMPSON JR,
JELKMANN W, SCHOEN CD. Detection and tentative grouping of
Strawberry crinkle virus isolates. European Journal of Plant Pathology 110
(1): 45-52, 2004.
•
VASKOVA D, SPAK J, KLERKS MM, SCHOEN CD, THOMPSON JR,
JELKMANN W.: Real-time NASBA for detection of Strawberry vein banding
virus. European Journal of Plant Pathology 110 (2): 213-221, 2004.