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αLA Coomassie E1 αKGDH E2 PDHC LipS E3 αKGDH and PDHC E2 αKGDH LipS in vitro activity. Recombinant LipS was purified and assayed in the presence of protein substrate and cofactor Sadenosylmethionine (SAM). The product of SAM cleavage (5’ dA) was monitored over the course of LipS reaction. 5' dA Produced by LipS H Protein 70 Malaria LipS binds to the 3 lipoate-dependent enzyme complexes found in E. coli. The labeled bands were identified by amino acid sequencing and/or anti-lipoate Western blot. Tight LipS/substrate complexes will now be used to stabilize LipS, and to characterize the mechanism guiding substrate specificity. 5' dA (uM) 60 50 40 30 20 10 0 0 5 10 Time (min) 15