Download Thin Layer Chromatography (TLC)

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[5] Measurement of chromatograaphic
data
• [i] Making permanent record
• most sprays produce coloured spots
• check for extra spots under UV light
– mark with needle point
• trace all plates with pencil
– use tracing paper same size as plate
– allow heated plates time to cool
• transfer data to paper record
• each drawing should be labelled with
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coating substance and activation period
thickness of layer
solvents used
spray used