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Chap 9. Cell Respiration Why is cell respiration so necessary? Living cells need a constant supply of energy • Examples: heart cells beating, muscle cells contracting, cells being made and repaired, etc. • Organisms take food & break it down into glucose (mostly in the small intestine) • cell respiration – process of breaking down glucose in the presence of O2 to release energy - Done by eukaryotes! Equation for Cell Resp. 6O2 + C6H12O6 → 6CO2 + 6H2O + energy • The energy that is released is used to make ATP How Does It Happen? • Glucose – simple sugar (C6H12O6) • Glucose is present in food • Food is broken down by our digestive systems • The chemical bonds in food are broken & the energy is released (Exergonic!) • 2 major types of organisms getting energy: 1. aerobic respiration. – with O2 2. anaerobic fermentation – without O2 Review of ATP-ADP Cycle Cell Respiration • Step 1 – Glycolysis: Takes place in Cytoplasm • A. Process of 1 molecule of glucose broken in half • B. Two 3-carbon compounds produced; each called pyruvic acid • C. 4 ATP molecules are produced • D. 2 ATP molecules are used for the reaction to occur • E. Net gain of ATP molecules = 2 Glycolysis – Cont. • Each electron carrier (NAD+ or uncharged battery) accepts highenergy electrons from H & carries them to the next reaction • NADH is now the charged battery Review of Glycolysis Glycolysis • Converts 1 glucose (C6) to 2 pyruvic acids (C3) • Produces : 2 pyruvic acids 2 NADH 2 ATP (net) • Occurs in cytoplasm [anaerobic] –all organisms do glycolosis Aerobic Respiration Needs Mitochondria! • The Krebs Cycle and Electron Transport chain take place here! Cell Respiration AEROBIC! • Step 2 – Krebs Cycle (Citric Acid Cycle) • A. Pyruvic acid is broken down in a series of reactions • B. Also called the citric acid cycle b/c citric acid is the 1st compound produced • C. CO2, 8NADH, 2FADH2 & 2 ATPs are produced • Takes place in the mitochondria matrix • The matrix is inside the inner membrane • http://highered.mheducation.com/sites/0072507470/student_view0/cha pter25/animation__how_the_krebs_cycle_works__quiz_1_.html Cell Respiration AEROBIC! • Step 3 – Electron Transport Chain • Takes place in the mitochondria inner membrane • Uses high-energy electrons carried from NADH + FADH2 in the Krebs Cycle • The ETC produces 32 ATP molecules, H2O, and un-charges electron carriers Electron Transport Chain (ETC) - Uses high-energy electrons from the Krebs cycle to convert ADP into ATP. - Occurs along the inner membrane of the mitochondria. - The electrons from NADH & FADH2 are passed along a series of proteins. - This movement of electrons pumps H+ ions into the inner membrane space. - The H+ ions will move from high to low concentration through the enzyme ATP Synthase. - This movement causes the enzyme to move like a turbine and bind ADP to P forming ATP. http://highered.mheducation.com/olcweb/cg i/pluginpop.cgi?it=swf::535::535::/sites/d l/free/0072437316/120071/bio11.swf::Elec tron%20Transport%20System%20and%2 0ATP%20Synthesis Why does it need oxygen? Oxygen is the final electron acceptor! Electron Transport Chain Electron Transport Chain • Where are the electrons moving?__________________ • Where are the H+ ions in a high concentration?_______ • When the electrons are finished going through the chain, what is the final electron acceptor?________, with H+ ions, it turns into ________. • The enzyme that make ATP is _________________. Totals of Aerobic Respiration - With aerobic respiration, a total of 36 ATPs are produced per glucose. Comparing Photo & Cell Respiration Photosynthesis Cell Respiration Energy goes in and is stored Occurs in chloroplasts Energy comes out Raw materials – CO2 & H2O Raw materials – glucose & O2 Products – glucose & O2 Products – ATP, CO2 & H2O Occurs in mitochondria Review of Glycolysis Glycolysis • Converts 1 glucose (C6) to 2 pyruvic acids (C3) • Produces : 2 pyruvic acids 2 NADH 2 ATP (net) • Occurs in cytoplasm [anaerobic] Cell Respiration When O2 is not present gylcolysis is followed by a different path • A. Fermentation The release of energy from food in the absence of O2 – It allows the cells to continue glycolysis because it frees up NAD+ (NADH -> NAD+) • B. Two types: • C. alcoholic fermentation • D. lactic acid fermentation Types of Fermentation 1. Alcoholic Fermentation • A. NADH is converted back to NAD+ and energy • B. Occurs in yeast cells; used in baking bread (CO2 makes bread rise) & in making alcohol • equation: pyruvic acid + NADH → alcohol + CO2 + NAD+ Types of Fermentation 2. Lactic Acid Fermentation – • A.Occurs in muscle cells when they run out of O2 • B.Causes pain & soreness • equation: • pyruvic acid + NADH → lactic acid +NAD+ Also occurs in bacteria cells, we use it to make yogurt, sauerkraut, and kimchi One more thing! • The only way to get rid of your lactic acid after a hard workout is through a pathway that requires extra oxygen. • That is why you breathe heavy after working out – to repay the oxygen debt.