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Supplementary Data 1 Miyagi et al. 60 r=-0.702** 0 2 4 6 8 40 20 r=0.389 0 0 2 4 6 8 Itaconate (µmol/gFW) 10 mM Itaconate 1 weeks Ascorbate (µmol/gFW) Isocitrate (µmol/gFW) Oxalate (µmol/gFW) 12 10 8 6 4 2 0 Citrate (µmol/gFW) New Leaves 0.12 0.10 0.08 0.06 0.04 0.02 0.00 r=0.052 0 2 4 6 8 2.0 1.5 1.0 0.5 r=0.195 0.0 0 2 4 6 8 Itaconate (µmol/gFW) 10 mM Itaconate 2 weeks 10 mM Itaconate 3 weeks -2 -3 -2 -1 b -2 c 3 -1 PC 2 (18.1 %) 0.06 2 -0.02 1 0.00 0 PC 1 (35.1 %) -0.04 0 1 3 -1 0 0 1 2 1 3 -1 PC 3 (10.4%) 2 1 0 -1 2 PC 3 (10.4%) 2 1 0 2 -1 Control 0 week 10 mM Itaconate 0 week -2 3 -0.06 e 0.10 0.08 0.06 0.04 0.02 0.00 -0.02 PC 1 (35.1 %)-0.04 -0.06 3 -0.08 -2 f 0.20 -0.15 Fumarate Malate Gln G1P Oxalate 3PGA PEP 2OG His Pro Met Thr Arg Asn Ile Val Lys Leu Phe Tyr GABA Citrulline Cinnamate Glu R5P Ru5P G6P DHAP Ala Ser Trp Cys Citrate Succinate Pyruvate Shikimate Asp FBP Gly Aconitate 6PG Coumarate RuBP Ascorbate Ornithine Isocitrate 3 Fumarate Malate Gln G1P Oxalate 3PGA PEP 2OG His Pro Met Thr Arg Asn Ile Val Lys Leu Phe Tyr GABA Citrulline Cinnamate Glu R5P Ru5P G6P DHAP Ala Ser Trp Cys Citrate Succinate Pyruvate Shikimate Asp FBP Gly Aconitate 6PG Coumarate RuBP Ascorbate Ornithine Isocitrate a Fumarate Malate Gln G1P Oxalate 3PGA PEP 2OG His Pro Met Thr Arg Asn Ile Val Lys Leu Phe Tyr GABA Citrulline Cinnamate Glu R5P Ru5P G6P DHAP Ala Ser Trp Cys Citrate Succinate Pyruvate Shikimate Asp FBP Gly Aconitate 6PG Coumarate RuBP Ascorbate Ornithine Isocitrate Stems Supplementary Data 2 Miyagi et al. d PC 1 loadings 0.04 0.02 Control 1 week Control 2 weeks 10 mM Itaconate 1 weeks 10 mM Itaconate 2 weeks : Organic acid : Amino acid : Phosphorylated compound -2 PC 2 loadings PC 3 loadings 0.15 0.10 0.05 0.00 PC 2 -0.05 (18.1 %) -0.10 Control 3 weeks 10 mM Itaconate 3 weeks Supplementary Data 3 Miyagi et al. 3 r=-0.113 2 1 0 Citrate (µmol/gFW) 0 1 2 3 4 60 40 20 r=-0.058 0 0 1 2 3 4 Itaconate (µmol/gFW) 10 mM Itaconate 0 week 10 mM Itaconate 1 weeks Ascorbate (µmol/gFW) Isocitrate (µmol/gFW) Oxalate (µmol/gFW) Stems 0.04 r=-0.488* 0.03 0.02 0.01 0.00 0 1 2 3 4 4 3 r=-0.396 2 1 0 0 1 2 3 4 Itaconate (µmol/gFW) 10 mM Itaconate 2 weeks 10 mM Itaconate 3 weeks
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