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Transcript 
Protein Synthesis
Translation Overview
One codon
One amino acid
Transfer RNA
Ribosome
Peptide bond formation
Codon-anticodon
matching
Transcription/Translation
T A C G G C A A A C T C C G G A C T
A U
T G C C G U
T U
T U
T G A G G C C U
T G A
Aminoacyl
Site
Peptidyl Site
Met
Phe
Pro
Exit Site
E P A
U A C G
A G
A C
A
A U G C C G U U U
Met
Pro
Phe
Pro
E P A
G G C A A A
A U G C C G U U U U A G
Wobble
Only 45 different
tRNA molecules exist
Pairing of the
anticodon with the
third base of the
codon is less specific
Protein Processing
Free
Bound
Polypeptide to
be used in
cytosol
Polypeptide is
headed to
membrane or
beyond via ER
Ribosome type depends
on ultimate destination
of polypeptide
Protein Processing
Signal Peptide
Cytosol
Rough ER
Receptor
Protein
DNA Function Review
1. What are the three sites in a ribosome?
2. Spliceosome:Intron::_____:_____
3. How is location of translation related to
the eventual use of its product?
4. What is wobble?
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