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Chapter 6 Proteins and Amino Acids Sources of Protein in the Diet Protein deficiency is rare in the United States. In the US, about two-thirds of dietary protein comes from meat, poultry, seafood, eggs and dairy products. Most of the world relies on plant proteins from grains and vegetables. As a country’s economy improves, the proportion of animal foods in the diet tends to increase. Copyright 2010, John Wiley & Sons, Inc. Nutrients Supplied by Plant and Animal Foods Animal products provide sources of protein, B vitamins and minerals such as iron, zinc and calcium. However, animal products are low in fiber and can be high in fat. Plant sources of protein are also a good source of B vitamins, iron, zinc, fiber and calcium, but in less absorbable forms. Copyright 2010, John Wiley & Sons, Inc. Amino Acids Amino acids are the building blocks of protein. Each amino acid contains a central carbon atom bound to a hydrogen atom an amino group an acid group a side chain. Copyright 2010, John Wiley & Sons, Inc. Copyright 2010, John Wiley & Sons, Inc. Protein differs from carbohydrate and lipid in that protein a) does not provide the body with a source of energy. b) is classified as an inorganic molecule. c) contains the element nitrogen. d) is not considered to be an essential nutrient. Copyright 2010, John Wiley & Sons, Inc. •Essential amino acids cannot be synthesized by the human body in sufficient amounts to meet needs. •Essential amino acids must be included in the diet. Copyright 2010, John Wiley & Sons, Inc. Amino Acids Copyright 2010, John Wiley & Sons, Inc. Amino Acids: Transamination When a nonessential amino acid is not available from the diet, it can be made in the body by the process of transamination. Copyright 2010, John Wiley & Sons, Inc. Protein Structure Peptide bonds are chemical bonds that link amino acids together. Peptide bonds are formed between the acid group of one amino acid and the nitrogen group of the next amino acid. Dipeptide bonds are formed between two amino acids. Polypeptides are formed between many amino acids. A protein is made of one or more polypeptide chains folded into a three-dimensional shape. Copyright 2010, John Wiley & Sons, Inc. Protein Structure Copyright 2010, John Wiley & Sons, Inc. 7. Which of the following terms can be used to describe a protein? a) polypeptide. b) monopeptide. c) polysaccharide. d) glycogen. Copyright 2010, John Wiley & Sons, Inc. Protein Shape Determines Function The final shape of a protein determines its function. Connective tissue proteins and collagen are elongated. Hemoglobin has a spherical shape. If the shape of a protein is altered, its function may be disrupted. Copyright 2010, John Wiley & Sons, Inc. If Protein Shape is Altered, Function May Be Altered Copyright 2010, John Wiley & Sons, Inc. If the shape of a protein is altered, the ___________ of the protein is altered. a) nutritional value b) functionality c) taste d) smell Copyright 2010, John Wiley & Sons, Inc. Protein Digestion and Absorption Copyright 2010, John Wiley & Sons, Inc. The enzymes trypsin and chymotrypsin break large polypeptides into smaller peptides. are needed to denature proteins. rearrange the sequence of amino acids in a polypeptide. are needed to make nonessential amino acids. Copyright 2010, John Wiley & Sons, Inc. Amino Acids Copyright 2010, John Wiley & Sons, Inc. Proteins that May Harm Certain Individuals: Phenylketonuria PKU is an inherited condition attributed to a defective gene. Aspartame, a sugar substitute, contains phenylalanine. Copyright 2010, John Wiley & Sons, Inc. Protein Digestion and Food Allergies Food allergies are triggered when a protein from the diet is absorbed without being completely digested. Proteins from milk, eggs, nuts, wheat, soy, fish and shellfish and peanuts are common causes of food allergies A rapid, severe allergic reaction is called anaphylaxis. Copyright 2010, John Wiley & Sons, Inc. Amino Acid Pool Allows for Amino Acid Availability Copyright 2010, John Wiley & Sons, Inc. Protein Synthesis: Transcription and Translation Copyright 2010, John Wiley & Sons, Inc. The sequence of amino acids in a polypeptide chain is determined by a) the types of food a person eats. b) the genetic material DNA. c) digestive enzymes called proteases. d) the hormone insulin. Copyright 2010, John Wiley & Sons, Inc. Energy Production from Protein Copyright 2010, John Wiley & Sons, Inc. Protein Functions Examples of proteins Enzymes speed up metabolic reactions (ie: trypsin) Transport proteins move substances in and out of cells (ie: hemoglobin) Antibodies help the immune system in fighting off foreign bodies. Contractile proteins help the muscles to move. Hormones are chemical messengers, such as insulin and glucagon. Proteins help to regulate fluid and acid-base balance. Copyright 2010, John Wiley & Sons, Inc. Protein Deficiency Protein-energy malnutrition (PEM) is a term that covers a range of protein deficiency conditions that may include only protein deficiency or protein deficiency plus energy deficiency. Kwashiorkor is a pure protein deficiency. Marasmus is an energy deficiency. Copyright 2010, John Wiley & Sons, Inc. Kwashiorkor is a pure protein deficiency Copyright 2010, John Wiley & Sons, Inc. Marasmus is an energy deficiency. Copyright 2010, John Wiley & Sons, Inc. Protein Excess Elevated protein intakes over long periods of time can result in: Hydration and kidney function issues Bone health issues Increased risk of heart disease and cancer Copyright 2010, John Wiley & Sons, Inc. Nitrogen Balance Copyright 2010, John Wiley & Sons, Inc. Determining Protein Requirements Copyright 2010, John Wiley & Sons, Inc. Calculating Protein Requirements Copyright 2010, John Wiley & Sons, Inc. One reason to get more of your protein from plants would be? to get more fiber to get more poly and mono unsaturated fats to lower blood cholesterol All answer choices are correct. Copyright 2010, John Wiley & Sons, Inc. How do you meet your needs if you are a vegetarian? Copyright 2010, John Wiley & Sons, Inc. Using MyPyramid for Daily Protein Selection Copyright 2010, John Wiley & Sons, Inc. Meeting Protein Needs with a Vegan Diet Copyright 2010, John Wiley & Sons, Inc. Considering Protein Quality Copyright 2010, John Wiley & Sons, Inc. Considering Protein Quality Copyright 2010, John Wiley & Sons, Inc. Meeting Protein Needs with a Vegetarian Diet Copyright 2010, John Wiley & Sons, Inc. Meeting Nutrient Needs with a Vegan Diet Copyright 2010, John Wiley & Sons, Inc. Food allergies involve an interaction between a protein from the diet and the immune system. the excretory system. the genetic material DNA. the genetic material RNA. Copyright 2010, John Wiley & Sons, Inc. The amino acid pool refers to amino acids a) which are soluble in bodily fluids. b) present in body tissues and fluids that are available for protein synthesis. c) d) amino acids which are used by the body for energy. which the body is not able to make and must be supplied by the diet. Copyright 2010, John Wiley & Sons, Inc. Chapter 6 Copyright 2010 John Wiley & Sons, Inc. All rights reserved. Reproduction or translation of this work beyond that permitted in section 117 of the 1976 United States Copyright Act without express permission of the copyright owner is unlawful. Request for further information should be addressed to the . Permissions Department, John Wiley & Sons, Inc. The purchaser may make back-up copies for his/her own use only and not for distribution or resale. The Publisher assumes no responsibility for errors, omissions, or damages caused by the use of these programs or from the use of the information herein. Copyright 2010, John Wiley & Sons, Inc.