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The Application of Biosensors in Monitoring Internal Cooking Temperature in Poultry Products Fenghua Jin Bio-imaging & Machine Vision Laboratory University of Maryland Outbreak Alert! (CSPI report, 2001) Introduction • Foodborne outbreaks: 76 million cases foodborne illness occurs and 5,000 dead annually (Morbidity and Mortality Weekly Report, 1999). • Cost of foodborne diseases is considerable — Annual cost of medical treatment and productivity lost $1.8 to $4.8 billion (Agricultural Economic report, 1996). Introduction (cont.) • most outbreaks are linked to inadequate cooking of meat and poultry products Handling the food with sufficient cooking • What does the sufficient mean? — Undercook bacteria would survive and threaten consumers’ health — Overcook the bacteria are cooked away, as well as meat tenderness and juiciness (USDA: Chicken breast: 76.7oC ) Problem • How to confirm that the internal cooking temperatures of meat products have reached the requirements? Methods to Measure Internal Cooking Temperature • One simple Method: Using Thermocouple —Thermocouple is the most widely used temperature sensor. What are thermocouples and how do they work? • Any two wires of different materials can be used as a thermocouple if connected together • TJct : Junction temperature • TRef: Reference temperature • E : A low-lever DC voltage (depends on the materials A and B, TJct , and TRef ) What are thermocouples and how do they work? (cont.) • Thermocouple materials —The three most common thermocouple alloys are: 1. Iron-Constantan (Type J) 2. Copper-Constantan (Type T) 3. Chromel-Alumel (Type K) * The first named element of the pair is the positive element and the negative wire is color coded red (current U.S. standards) What are thermocouples and how do they work? (cont.) • Establish Reference Temperature — Ice Baths accurate and inexpensive — Electronically Controlled References not as accurate as ice-baths but convenient What are thermocouples and how do they work? (cont.) • Circuit — The signal is brought out of the reference temperature region to a voltmeter at room temperature. This is done using a pair of copper wires. Limitation of the thermocouple • It is sporadic and does not ensure that all portions of the meat reach the required cooking temperature. • The process is slow, invasive and susceptible to cross-contamination. Need new approaches to measure the internal cooking temperatures of poultry meat Infrared Imaging in Food Analysis • What is Infrared Imaging? — Infrared means "below red" and has longer wavelength than red light. — Infrared light is invisible to the unaided eye, but can be felt as heat on one’s skin. — Warm objects emit infrared light. electromagnetic spectrum Infrared Imaging in Food Analysis (cont.) • The theoretical foundation behind this technology is described by Stefan-Boltzmann law: E T 4 — E is the radiation intensity of an emitter — σ is Stefan-Boltzmann constant —ε is emissivity, a material property of the object — T is the absolute surface temperature of the object E depends on the fourth power of T Infrared Imaging in Food Analysis (cont.) • Sequential Infrared images: Temperature (oC) 95 thermocouple 83 (1) 0 70 (2) 10 (i) Time (second) 57 47 Advantage of Infrared Imaging 1. can measure the surface temperature of solid materials for which it is difficult to insert the probe; 2. can measure the temperature of some material that can erode the probe or reduce its usage time; 3. nondestructive, high-speed, automatic monitoring and accuracy Infrared Imaging in Food Analysis (an example) • Ibarra et al. (1999) developed a method using IR imaging to estimate the internal cooking temperature in chicken breast. The internal temperatures recorded by thermocouples (TCs). External temperatures was recorded by an IR camera Time series observations of simultaneous internal and external temperatures were obtained. Using Artificial Neural Network (ANN) to model the nonlinear heat transfer process and to predict the internal cooking temperature Infrared Imaging in Food Analysis (an example cont.) • Limitations: The chicken samples had similar shape and thickness — Variant shapes and thickness may directly affect the heat transfer process in meats. We need to extract 3D information of poultry meat — Laser range imaging is a well-established technique to get 3D information of the object. Laser Range Imaging laser projector camera (xi, yi) x lens (0, 0, 0) detector . (xs, ys, zs) f y z zref θx (xo, yo, zo) d object encoder pulse z z 1 xz d ref o i ref f x s ( Z ref Z o ) cos x Artificial Neural Network Modeling • Internal temperature • External temperature • 3D information of poultry meat use Artificial Neural Network to model the nonlinear heat transfer process and to predict the internal cooking temperature Introduction to Artificial Neural Network hidden layer input layer output layer wij bi Inputs: p Outputs: a node • Two procedures of ANN modeling: — Learning — Testing Learning Process Initialize W, b a p f W a b aa 0 a m 1 m 1 m 1 m m 1 M Calculate E Stop Y E=0 E < Emax E: Performance index; N Emax: Performance goal. W = W + ∆W b = b + ∆b * performance of network is defined by performance index e.g.: mean square errors (MSE) Two procedures of ANN modeling • Training weights and biases of the ANN were determined by the ANN training process; . • Testing The surface temperature and the geometric information were inputted into the ANN to predict the internal cooking temperature in chicken samples. Illustration of the IR and laser range imaging system IR camera Laser projector Oven CCD camera Oven Infrared Camera Laser projector and CCD cameral T type thermocouple System block diagram ANN Modeling Endpoint Temperature Monitoring System Internal Temperature Recording IR Imaging Cooking System Laser Range Imaging Extended real-time endpoint temperature estimation system ANN Modeling Endpoint Temperature Monitoring System IR Imaging Laser Range Imaging Cooking System Decision System Control Unit