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Endoderm and primary induction Human Figure 11.32(1) Tissue Formation in the Human Embryo Between Days 7 and 11 Figure 11.32(2) Tissue Formation in the Human Embryo Between Days 7 and 11 Figure 11.33(1) Amnion Structure and Cell Movements During Human Gastrulation Huma Figure 1.6 Fate Maps of Different Vertebrate Classes at the Early Gastrula Stage Figure 1.5 The Similarities and Differences among Different Vertebrate Embryos START PRIMARY ORGANIZER LECTURE FROM HERE Terms (definitions) for Establishment of cells and tissues (here use lens cells as an example) • Competence, wherein cells can become lens precursors if they are exposed to the appropriate combination of signals. • Specification, wherein cells have received the appropriate signals to become lens precursors, but progression along the pathway to lens can still be repressed by other signals. • Commmitment (determination), wherein lens precursors have entered a differentiation pathway, and will become lens even in the presence of inhibitory signals. • Differentiation, wherein the lens cells leave the mitotic cycle and express those genes characteristic of their cell type. Early dpp/BMP gradients dictate the D/V axis of the entire embryo Early dpp/BMP gradients dictate the D/V axis of the entire embryo Figure 23.14 Homologous Pathways Specifying Neural Ectoderm in Protostomes (Drosophila) and Deuterostomes (Xenopus) D/V Later cases of dpp/BMP expression direct specific embryonic tissue inductions Later cases of dpp/BMP expression direct specific embryonic tissue inductions Later cases of dpp/BMP expression direct specific embryonic tissue inductions Sea Urchin BMP-2/ BMP-4 Nodal Figure 3.14 Roux’s Attempt to Demonstrate Mosaic Development Wilhelm Roux, 1888 Figure 3.11 Conditional Specification Two critical inductions: Formation of the primary organizer Action of the primary organizer Neurulation is induced (By the ‘primary organizer’) -1. The primary organizer induces neurulation and axis formation -2. The primary organizer itself arises from a previous induction -1. The primary organizer induces neurulation and axis formation -The dorsal lip of the blastopore contains the primary organizer -1. The primary organizer induces neurulation and axis formation -The dorsal lip of the blastopore contains the primary organizer -1. The primary organizer induces neurulation and axis formation -The dorsal lip of the blastopore contains the primary organizer -1. The primary organizer induces neurulation and axis formation Figure 10.21(1) Organization of a Secondary Axis by Dorsal Blastopore Lip Tissue -1. The primary organizer induces neurulation and axis formation Figure 10.21(1) Organization of a Secondary Axis by Dorsal Blastopore Lip Tissue Amphibian -1. The primary organizer induces neurulation and axis formation Source of primary organizer shown on fate map Amphibian -The primary organizer induces neurulation and axis formation -The primary organizer itself arises from a previous induction -The primary organizer itself arises from a previous induction OR . . . where does the ‘primary organizer’ come from? -The primary organizer itself arises from a previous induction Experiment at the 32-cell stage Contains the Nieuwkoop center -The primary organizer itself arises from a previous induction The primary organizer is induced by the Nieuwkoop center (a tissue with an endodermal fate) The primary organizer arises due to an induction from the Nieuwkoop center How does the Nieuwkoop center arise? Spemann, 1938 - baby’s hair ligature The primary organizer arises due to an induction from the Nieuwkoop center How does the Nieuwkoop center arise? The primary organizer arises due to an induction from the Nieuwkoop center How does the Nieuwkoop center arise? The primary organizer arises due to an induction from the Nieuwkoop center How does the Nieuwkoop center arise? The primary organizer arises due to an induction from the Nieuwkoop center How does the Nieuwkoop center arise? Contains the Nieuwkoop center Figure 11.18 Formation of the Nieuwkoop Center in Frogs And Chicks Figure 11.19 Formation of Hensen’s Node From Koller’s Sickle Figure 11.20(1) Induction of a New Embryo by Transplantation of Hensen’s Node THE ORGANIZER IS IN THE HENSEN’S NODE Mammal Zebrafish Figure 11.7 Convergence and Extension in the Zebrafish Gastrula Zebrafish Figure 11.8 The Embryonic Shield as Organizer in the Fish Embryo Figure 1.6 Fate Maps of Different Vertebrate Classes at the Early Gastrula Stage All have homologous organizers Figure 1.5 The Similarities and Differences among Different Vertebrate Embryos