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Photosynthesis State Objectives! CLE 3210.3.3 Investigate the relationship between the processes of photosynthesis and cellular respiration SPI 3210.3.3 Compare and Contrast photosynthesis and cellular respiration in terms of energy transformation. Introduction: Overview 6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2 Photosynthesis consists of two independent pathways light-dependent reaction (light reaction) the light-independent reaction (dark reaction). Introduction Light Reactions: the energy in sunlight is trapped, O2 is released, and both ATP and NADPH + H+ (hydrogencarrier molecule) are formed Dark Reactions: the ATP and NADPH + H+ react with CO2 from the atmosphere and form glucose The entire process results in the transformation of light energy from the sun into energy stored in the bonds of the glucose molecule. Structure of a Chloroplast The light reactions take place in the thylakoid membrane The dark reactions take place in the stroma Structure of a Chloroplast Electromagnetic Spectrum Why are plants green? Pigments Pigments are light-absorbing compounds. Pigments appear colored because they absorb light of certain wavelengths and reflect that of others. Chlorophyll a is the primary pigment in green plants that absorbs red and blue/violet light and reflects green light. Structure of Chlorophyll Why aren’t these leaves green?? Accessory Pigments Chloroplasts also contain other pigments called accessory pigments. Accessory pigments trap wavelengths of light that cannot be absorbed by chlorophyll a and then transfer the energy to chlorophyll a molecules for use in photosynthesis. In this way, accessory pigments enable plants to use a greater amount of the sun’s energy than is available to chlorophyll alone. Light Reaction Light Reaction Also called Light Dependent Reaction Pigments that are in the chloroplasts intercept light and begin the light reactions of photosynthesis. The light reactions occur in two photosystems (located in the thylakoid membrane): -photosystem I (PSI) -photosystem II (PSII) Photosystem: a unit of several hundred chlorophyll a molecules and associated acceptor molecules Photosystems Excited Electrons NADPH NADP+ is an electron acceptor NADP+ + 2 e- + 2 H+ NADPH + H+ Primary Electron Acceptor ETC Proton Pump PSI PSII Lumen Thylakoid Water (H2O) Oxygen (O2) ATP Synthase Hydrogen Ion (H+) Electron Step 1 Step 2 Step 3 Step 3 Step 3 Step 3 Step 3 Step 3 ~ e low high Step 4 Step 4 Step 4 Step 4 Step 4 Step 4 Step 5 Step 5 Step 5 Step 5 Step 5 NADP+ NADPH + H+ Step 5 high low Step 6 Step 6 Step 6 Step 6 Step 6 ADP + P ~ Step 6 e ATP NADP+ ADP + P Reactants O2 is released into the atmosphere NADPH + H+ DR Products ATP Dark Reaction Dark Reaction The dark reaction is also known as the Calvin Cycle, after an American scientist (Melvin Calvin) who figured out the pathway. The dark reaction is also known as the C3 Cycle because the first stable products of this pathway are molecules that contain three carbon atoms. The Calvin Cycle occurs in the stroma. 6 CO2 C 6 RuBP C C C C C 6 ATP C C C ~ 12 PGA e 6 ADP + P 12 ATP ~ e 12 ADP + P C C C C C C Glucose C C C 12 PGAL C C C e- C4 vs. CAM WHY?? The End!