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s- Fig 3 D A G * ** ** ** ** H E B ** ** F C ** I ** ** ** * Figure S3. an3 Plants Possess Reduced Every Water Loss and Improved WUE as a Result of Lower Transpiration and Stomatal Conductance. (A) Every water loss of 6-week-old wild-type (Col-0) and an3 plants grown soil under normal conditions was assayed (mean ± SD, n= 3; **P < 0.01). Shoot dry weight (B) was the mean ± SD (n = 3). (C) Integrated WUE of wild-type and an3 plants under well water conditions was determined from gravimetric measurements of water loss and shoot dry weight during 6 weeks (mean ± SD, n=10; **P < 0.01). (D) Leaf transpiration ratio, (E) instantaneous WUE, (F) stomatal conductance, (G) internal CO2 concentration, (H) net CO2 assimilation, and (I) VPD were assayed on individual leaves of 6-week-old wild-type (Col-0) and an3 plants using a Li-Cor 6400 gas exchange system (mean ± SD, n= 3 **P < 0.01; *P < 0.05). For obtaining well developed and largened leaves, we irrigated plants using 1/15 MS liquid.