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Expression Data Generation and
Collection at MPIMG and RZPD
Resource Center within the
German Human Genome Project
Steffen Schulze-Kremer
Background History
 1987
 first spotting system (EMBL,ICRF)
 ~1989
 complex cDNA Hybridisations to arrays
 ~1989 onward
 distribution of arrays in reference library
system
RZPD Database Objectives (since 95)
 To support dissemination of variety of
biological materials
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Clones
Filters
Pools
 To collect and redistribute biological
primary data
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Hybridisations
Sequences
Phenotypes
RZPD Database Coverage
 Biological Information
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Clones
Libraries
Genes
Sequences
Hybridisation
 Logistic Information
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Clone storage
Library re-arraying
Filters
 Accounting Information
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Customers
Orders / requests
Affiliation (DHGP?)
Price lists
 Information Integration
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Confidential information
Links to other databases
Query
Visualisation
RZPD Database Contents (Oct 5/99)
 Biological Information
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27.582.866 clones
• 17.280.664 master
clones
319 libraries
47.209 filters
581.219 sequences
24.366 hybridisations
352.806 Blast hits
• 187.952 distinct
targets hit
 Accounting Information
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5551 registered users
1228 organisations
• 630 German
• 598 foreign
# RZPD orders (in RLDB)
• 15.980 clones (4405)
• 3610 filters (1468)
• 1118 pools (0)
 Submissions
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38.042 sequences (DHGP)
15.589 sequences (other)
Graphical Filter Display Tool
Database Components
 Oracle / Unix
 DEC Alpha 8 Processor Server
 Secure Web Server
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protects confidential information during transit
different roles available
customised views
 OPM Tools
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some 80 object classes
class browser
query editor
OPM Schema Browser
Hybridisation Data in RZPD
Laboratory Database at MPIMG
Dictionaries
„Owned Objects“
„Result“
„Experiment“
 Lab Database Development Team at MPIMG

Dr. Arif Malik, Axel Nagel, Dr. Holger Eickhoff
RZPD Distribution Service, Database &
Bioinformatics Team (www.rzpd.de)
 Distribution Service

Nadja Pohl, Eva Partsch,
Patience Frederick,
Maryam Sabet
 Registration

Radka Kochan
 Database Managers
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Karsten R. Heidtke,
Peter Neubert
 Web Designer
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Iris A. Bertram
 System Administration

Klaus Dobrindt, Robert Fürst
 Application Programmers
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Elisabeth Maschke,
Ralf Mühlhaus
 Data Submission
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Dr. Andreas Hewelt
 Students
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Falco Ripsas, Katja Wegner
 Protein Structure Factory
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Dr. Jörn Quedenau,
Ursula Meyhoefer
 Informatics Manager
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Dr. Steffen Schulze-Kremer