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CYTOLOGY THE STUDY OF CELLS STRUCTURE GENERAL Cell = Smallest Living Unit Produced by Cell Division or Fertilization Surrounded by Interstitial / Extracellular Fluid Components: - Plasma (Cell) Membrane - Cytoplasm - Organelles THE PLASMA MEMBRANE Phospholipid bilayer: Hydrophobic tails and hydrophilic heads, selectively permeable Proteins: Peripheral and integral Enzymes Receptors Transport Anchors Carbohydrates: Superficial recognition sites THE PLASMA MEMBRANE cont. - Fluid Mosaic Model (mosaic of floating proteins) - Functions of Plasma Membrane: * Physical Barrier (Selective!) * Regulates Exchange with Environment * Sensitive to Extracellular Environment * Structural Support THE PLASMA MEMBRANE CYTOPLASM - Material Between Plasma Membrane & Nucleus - Components * Cytosol (water + nutrients & other solutes) * Organelles (excludes nucleus) * Inclusions (stored materials) - Functions: Site of chemical reactions ORGANELLES “Little Organs” Membranous organelles - Membrane isolates secretions, enzymes or toxins Non-membranous organelles NUCLEUS (Membranous) Double Nuclear Membrane with Pores Nucleoplasm - Nucleolus * Organelle in nucleus (non-membranous) * Assembles ribosomes - DNA * Chromatin - loose; protein synthesis * Chromosomes - dense, rodlike; cell division STRUCTURE OF THE NUCLEUS ENDOPLASMIC RETICULUM (Membranous) Smooth endoplasmic reticulum (SER) - Synthesis of Carbohydrates, Steroids & other Lipids - Storage (Synthesized Materials or Ca+) Rough endoplasmic reticulum (RER) - Attached Ribosomes - Protein synthesis for “export” or inclusion in cell membrane GOLGI COMPLEX/APPARATUS (Membranous) Structure: - Flattened Membranous Sacs Function: - Modifies & packages proteins - Source of lysosomes - Renewal/modification of plasma membrane MITOCHONDRIA (Membranous) Structure: Double membrane Function: Production of ATP (95%), cellular energy Contains DNA Self-Replicating MITOCHONDRIA LYSOSOMES (Membranous) Structure: Vesicles filled with Digestive Enzymes (“Suicide Bags”) Function: - Digestion within the cell - Defense - Recycle worn-out cells Peroxisomes Membranous sacs of enzymes Detoxify harmful/dangerous substances - Alcohol - Formaldehyde - Free Radicals VACUOLES AND VESICLES (Membranous) Structure: Membranous Sac Function: Storage, Endocytosis, Exocytosis FORMATION OF VESICLES RIBOSOMES (Nonmembranous) Structure: Two subunits (rRNA + protein) Function: Protein synthesis Fixed or Free CENTROSOMES AND CENTRIOLES (Non-membranous) Centrosome: “Cell center” - Area of cytoplasm - Microtubule formation center - Contains Centrioles Centrioles: - Microtubules - Form Mitotic Spindle CYTOSKELETON (Nonmembranous) Network of protein structures Acts as cell’s “muscles & bones” Determines cell shape Supports organelles Involved in internal transport & cellular movements CILIA & FLAGELLA (Nonmembranous) Cilia – short, numerous, move substances over free surfaces Flagella – long, singular, move cell Microvilli – thin folds of cell membrane, increase surface area to maximize absorption