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Chapter 7 Biology Discovery of the Cell In the mid-1600s, Anton van Leewenhook used a single lens light microscope to observe pond water and discovered a world of tiny microscopic organism in the water. In 1665, Englishman, Robert Hooke used an early compound light microscope to look at a thin slice of cork a plant material. •He observed thousands of tiny empty chambers, he called cells now considered to be the basic units of life Development of Cell Theory In 1838, German botanist, Matthias Schleiden concluded that all plants were made of cells. The next year, German biologists, Theodore Schwann stated that all animals were made of cells. In 1855, German physician Rudolf Virchow concluded that new cells could only be produced from existing cells Together the works of these scientist and others led to the development of the cell theory the fundamental concept of biology! The Cell Theory states: All things are composed of cells Cells are the basic units of structure and functions New cells are produced from existing cells Exploring the Cell In the 1940s, a new type of microscope, the electron microscope was invented The Electron Microscope uses beams of electrons instead of natural light to magnify images and can do so up to 500,000 x the actual size of the image The Electron Microscope was better than the Light Microscope because it allowed scientists to see the internal structures within the cell There are several different types of Electron Microscopes: Scanning Electron Microscope (SEM): scans the surface within the cell Fly leg Transmission Electron Microscope (TEM): allows scientists to see internal structures. Pine tree Scanning Tunneling Microscope (STM): uses electrons to investigate the atoms of the surface of molecules 2 Types of Cells With the development and use of the microscopes, scientists were able to discover 2 groups of cells that exists in living organisms all contain: ribosome DNA cell membrane cytoplasm 1. Prokaryotic Cells: cells that lack internal membrane-bound structures called organelles (little organs) 2. Eukaryotic Cells: cells that contain internal membranebound structures or organelles (has a nucleus) Discovery of the Nucleus Robert Brown, a Scottish scientist, was the first to observe as prominent structure in cells, called the nucleus Rudolf Virchow later concluded that this structure (nucleus) is responsible for cell division (reproduction) in living organisms 6 Kingdom System (into which living organisms belong): 1. Eubacteria-prokaryotic cells ex: E. coli (bacteria) 2. Archaebacteria- prokaryotic cells ex. Methanogens (bacteria) 3. Protista-eukaryotic cells ex. Amoeba, algae 4. Fungi- eukaryotic cells ex. Mushrooms, athlete’s foot 5. Plants- eukaryotic cells ex. Pine, flowers 6. Animals- eukaryotic cells ex. Sponges, humans NOTE: THE ONLY Prokaryotes ON EARTH ARE bacteriums (monerans) Typical Prokaryotic Cell DNA Cell membrane Typical Eukaryotic Cell Ch 7.2 Biology Notes Cell Parts (Organelles) •Prokaryotic cells (prokaryotes)-are cells that do not have a nucleus. They are the most ancient (oldest) cells on Earth. Many of these earliest cells do not use oxygen to respire. They used CO2 in the atmosphere and lived in water and produced O2 gas as a byproduct. Prokaryotes are ONLY found in the kingdom Monera. Eubacteria and Archaebacteria Domains. They are bacteria. Eukaryotic cells (eukaryotes)-are cells that have a nucleus. They are found in all the other kingdoms: Plantae, Animalia, Fungi, and Protista. They are thought to have evolved from two bacteria living together symbiotically (both benefiting). This is called the Endosymbiotic Theory. You have eukaryotic cells! Mitochondria and Chloroplast have own DNA. Nucleus-directs all activities of the eukaryotic cell. Surrounded by a nuclear envelope (membrane) Nucleolus-organelle inside the nucleus that make ribosomes Chromatin-is located in the nucleus of the cell; contains strands of DNA. Chromosomes are condensed chromatin. · · Plasma Membrane-a bilayer (2-layers) of phospholipids joined together to surround the cell. (Fatty acids + Phosphate Group) Primary Function is to maintain: Homeostasis Ribosomes-the site of protein synthesis. Ribosomes take amino acids and produce proteins using the instructions from DNA Cytoplasm-the fluid of the cell. Also called cytosol Where all chemical reactions of the cell takes place Endoplasmic Reticulum (ER)-are cellular pathways used to transport proteins Smooth ER=have no ribosomes Rough ER=have ribosomes Golgi Apparatus-sort, package, process proteins. They are the UPS of the cell. Produces little packages celled vesicles. Not connected to nucleus Vacuoles-in plant cells they are the largest structure. Responsible for storing large amounts of water. In animal cells, they are much smaller and more numerous. They store food, enzymes, and other materials needed by the cell. Sometimes they store waste products. Some have Contractile vacuole- to maintain Homeostasis. Lysosomes-recycle cellular parts. They can contain digestive enzymes that break down and reuse worn-out organelles and food particles. Chloroplasts-are cell organelles that capture light energy and convert it into chemical energy (glucose/food) for plant cells.. Chlorophyll is the green pigment used to trap light energy. Chlorophyll also gives plants their green color (belongs to a group called plastids). NOTE: The ultimate source of all energy on Earth is the SUN! Mitochondria-the site of cellular respiration (ENERGY). They transform food into energy in the form of ATP (adenosine triphosphate: a useable form of energy). Tissues or cells that use a lot of energy have large numbers of mitochondria. Muscle cells have more mitochondria than skin cells. Powerhouse of the cell. NCSCOS: mitochondria is folded to increase surface area (make more room). Organelle DNA Mitochondria and Chloroplast have own genetic DNA. It is suggested that mitochondria and chloroplast are descendents of ancient prokaryotes. Prokaryotes that use oxygen to generate ATP evolved into Mitochondria. Prokaryotes that carried out photosynthesis evolved into Chloroplast. Microtubules and Microfilaments-are thin, solid proteins fibers. Many anchor cilia (hair-like) and flagella (whip-like tail) that help one-celled organisms to move. Cell Wall-an inflexible barrier found in plants. It protects the cell and gives it support it is made up of cellulose and has porous fibers that allows molecules to pass through Centrioles-found only in animal cells. Aid(appears) in cellular division