Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
An insight into the tissue culture technique and its implication Dr.Samina Hyder Haq History of tissue culture 1885: Roux maintained embryonic chick cells in saline 1965:Ham introduced serum free culture medium that support living cells 1965: Harris & Watkins successfully fused human and mouse cells by virus 1975: Kohler & Milstein produced the first Hybridomas capable of secreting monoclonal antibodies. 1978: Sato developed a serum free medium with a cocktail of hormone and growth factors !982: human insulin is produced 1985: human growth factors were produced 1986: lymphoblastoid gamaIFNlicences 1987:tissue type plasminogen activator(tPA) became commercially available 1989:Recombinant erythropoitin in trial 1990:Recombinent products in trial (factorVI.HBsAg, Il2, Plant Tissue culture Micropropagation What is tissue culture Organ culture Explant Culture Cell culture Technique and instrument Carbon dioxide incubator Microscope Tissue culture Ware Culture Media Sterilization Cell counting Changing Medium Other miscellaneous Equipment Fridge Freezer for storing medium Liquid nitrogen Container to freeze certain cell lines Incubator for warming up of the medium Bench centrifuges to separate out cell pellet. Types of cell culture 1: Primary Cell culture 2: continuous Cell lines Secondary or continuous Cell Lines Attached Cell Lines Name Species and tissue of origin Morphology MRC-5 (Prod. No. 84101801) Human lung Fibroblast HELA (Prod. No. 93021013) Human cervix Epithelial VERO (Prod. No. 84113001) African Green Monkey Kidney Epithelial NIH 3T3 (Prod. No. 93061524) Mouse embryo Fibroblast L929 (Prod. No. 85011425) Mouse connective tissue Fibroblast CHO (Prod. No. 85050302) Chinese Hamster Ovary Fibroblast BHK-21 (Prod. No. 85011433) Syrian Hamster Kidney Fibroblast HEK 293 (Prod. No. 85120602) Human Kidney Epithelial HEPG2 (Prod. No. 85011430) Human Liver Epithelial BAE-1 (Prod. No. 88031149) Bovine aorta Endothelial SUSPENSION Cell lines Suspension Cell Lines Name Species and tissue of origin Morphology NSO (Prod. No. 85110503) Mouse myeloma Lymphoblastoid-like U937 (Prod. No. 85011440) Human Hystiocytic Lymphoma Lymphoblastoid Namalwa (Prod. No. 87060801) Human Lymphoma Lymphoblastoid HL60 (Prod. No. 98070106) Human Leukaemia Lymphoblastoid-like WEHI 231 (Prod. No. 85022107) Mouse B-cell Lymphoma Lymphoblastoid YAC 1 (Prod. No. 86022801) Mouse Lymphoma Lymphoblastoid U 266B1 (Prod. No. 85051003) Human Myeloma Lymphoblastoid SH-SY5Y (Prod. No. 94030304) Human neuroblastoma Neuroblast Storing and maintaining Continuous Cell Lines Advantages of Cell Culture Cell types kept Constant and homogenous. There is direct access to the cells so effect of toxicity of drug and chemicals studied without being lost to other tissues and excreted. Replace/ reduce the number of animals.Legal, moral, ethycal isses?(animal rights Group). Control of the environment (pH, osmotic pressure, temperature,oxygen and Carbondioxide tension.) Disadvantages of Cell Culture Contamination can be Chemical (culture medium) Or Biological (adding antibiotics). Finding a Happy Environment. De-differentiation Origin of Cells Major differences from in-vivo(loss of ECM) Implication for Cell Culture 1. Model system (many specialized functions are restored) 2.Toxicity Test (viability of cells) 3.Cancer research Virology Cell Based manufacturing Genetic councelling Genetic enginering Drug screening & development Gene Therapy Production of monoclonal antibodies Tissue Engineering Carbon nanotube scaffolding Name of Made up of scaffoldig Nanofiber Like carbon Textile Polyglycolide Gas Foam Foam like structure due to CO2 gas Growing cells in 3D Forming Tissue in Bioreactor Application of Monoclonnal Antibodies IMMUNOLOCALIZATION IMMUNOBlotting Cancer Treatment Immunolocalization Immunoblotting Cancer Treatment Stem cell research Embryonic Cell Lines Adult Cell Line Homeiopoitic Stem Cell Embryonic Cell lines Placental stem cell Placental stem cells do not possess the antigenic properties, making rejection of the stem cells impossible. It has successfully used in treating various neurological disorders like Parkinson, paralysis and stroke patients. Haematopoitic stem Cell Adult Mesenchymal Cell lines Organ transplant