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Ch. 3 Cells Cell Theory 1. Cells are the basic units of life 2. All organisms are made of one or more cells 3. Cells arise from other cells Figure 6.8a ENDOPLASMIC RETICULUM (ER) Flagellum Nuclear envelope Nucleolus Rough Smooth ER ER NUCLEUS Chromatin Centrosome Plasma membrane CYTOSKELETON: Microfilaments Intermediate filaments Microtubules Ribosomes Microvilli Golgi apparatus Peroxisome Mitochondrion Lysosome Cell Membrane Cell membrane – gate, controlling what comes in and out of the cells • Phospholipid bilayer – phosphate head and two fatty acid tails • Phosphate head – hydrophilic (water loving) • Fatty tails – hydrophobic (water hating) • Selectively permeable • Cholesterol – stability at high temperatures, flexibility at low temps. Proteins 1. 2. 3. 4. 5. 6. 7. Recognition proteins – identify the cell - glycoproteins Channel proteins – passage of small, hydrophilic substances in and out of the cell Adhesion proteins – form junctions with other cells Transport pumps – sodium/potassium pump Receptor proteins – G-protein linked Ion channels – Na/K channels Carrier proteins – move specific substances through membrane Prokaryote Cell – Bacterial Cells Prokaryote vs. Eukaryote Prokaryotes – bacterial cells • • • • Do not have a nucleus Have fewer parts, smaller ribosomes Have DNA and reproduce No membrane around organelles Eukaryotes – plants, animals • Have a nucleus • Have more parts • Membrane bound organelles Cytoplasm Cytosol and organelles Cytosol is mostly water Organelles – nucleus and other parts floating in cytosol Organelles Ribosomes – On the rough endoplasmic reticulum and free in the cytoplasm make proteins (manufacturer) Smooth endoplasmic reticulum detoxification and making lipids and carbohydrates Nucleus The control center – “brain” Nuclear envelope – covered in pores – send proteins out Contains DNA coiled around histone proteins (nucleosome) DNA coils into chromatin and chromatin condenses into chromosomes (blueprint) Nucleolus Dark structure inside the nucleus Make ribosomes Disappears during mitosis (cell division) Golgi and Lysosomes Golgi apparatus stack of pancakes Packages things and sends them out of the cell Lysosome digestion of food particles contain enzymes Low pH Mitochondria Powerhouse of the cell ATP – energy is created Cell Shape Cytoskeleton – internal framework in an animal cell that helps maintain cell shape. Microtubules – hollow tubes made of protein help maintain cell shape and serve as tracks for transport throughout the cell. Cillia and Flagella are made of microtubules. Microfilaments – smaller proteins that help maintain cell shape. Euglena Paramecium Vacuoles and vesicles Transport vesicles – move materials within the cells and through the endomembrane system Food vacuoles – temp. food storage, merge with lysosomes for digestive purposes Contractile vacuole – in some organisms for pumping water Central vacuole – in plants, fills with water creating turgor pressure Figure 6.32 Tight junctions prevent fluid from moving across a layer of cells Tight junction TEM 0.5 m Tight junction Intermediate filaments Desmosome TEM 1 m Gap junction Space between cells Plasma membranes of adjacent cells Extracellular matrix TEM Ions or small molecules 0.1 m Plant Cells Plant cells have a cell wall – made of cellulose They also have chloroplasts – where photosynthesis occurs Chlorophyll – pigment that absorbs sunlight energy Other functions of central vacuole – pg. 35 Plant Cells Cell Transport Semipermeable membrane – small, lipid-soluble particles can pass, but large, charged particles cannot Diffusion – net movement of particles from high to low concentration Concentration gradient – the difference in concentration between one area and another Cell Membrane Diffusion Osmosis Diffusion of water through aquaporins Moves from high water potential to low water potential. Hypertonic solution – higher solute concentration Hypotonic solution – lower solute conc. Isotonic soln. – solute conc. Equal on both sides and equilibrium. Osmosis Figure 36.9 Initial flaccid cell: 0.4 M sucrose solution: Plasmolyzed cell at osmotic equilibrium with its surroundings P 0 S 0.9 0.9 MPa P 0 S 0.9 0.9 MPa (a) Initial conditions: cellular environmental P 0 S 0.7 0.7 MPa Pure water: P 0 S 0 0 MPa Turgid cell at osmotic equilibrium with its surroundings P 0.7 S 0.7 0 MPa (b) Initial conditions: cellular environmental Facilitated Diffusion Passive transport – no Energy required Moves down concentration gradient Moves through a carrier or channel protein Ex. Glucose Lipid-insoluble substances Facilitated Diffusion Active Transport Not passive Goes up or against the concentration gradient (needs energy) Na+ out/K+ in Involves specific carrier proteins Important in muscle contractions, nerve impulses – maintains resting potential Ex. Plant roots, ion pumps Active Transport – Sodium/Potassium Pump Endocytosis Enodcytosis – cell taking materials in Pinocytosis – cell drinking Phagocytosis – cell eating Endocytosis Receptor-mediated endocytosis Exocytosis Releasing molecules into the extracellular matrix Bulk flow – passage of substances through vessels or xylem/phloem Exocytosis Countercurrent exchange Movement of materials in bulk flow in opposite direction Ex. Fish gills and capillaries Water(with fresh dissolved oxygen) entering gills encounter capillaries deficient in oxygen Diffusion of oxygen is enhanced Cell Junctions Plasmodesmata – tunnels between plant cells Junctions in animal cells – tight, anchoring, communicating