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http://www.williamsclass.com/Seve nthScienceWork/CellTheoryParts.h tm An animal cell Rough endoplasmic reticulum Smooth endoplasmic reticulum Nucleus Flagellum Not in most plant cells Lysosome Ribosomes Centriole Peroxisome Microtubule Cytoskeleton Intermediate filament Microfilament Golgi apparatus Plasma membrane Mitochondrion A plant cell Nucleus Rough endoplasmic reticulum Ribosomes Smooth endoplasmic reticulum Golgi apparatus Not in animal cells Microtubule Central vacuole Intermediate filament Chloroplast Microfilament Cell wall Mitochondrion Peroxisome Plasma membrane Cytoskeleton • Location: Plant – side of cell; Animal – Middle of Cell • Appearance: Bowl of spaghetti • Nickname: Control Center • Function: Initiates and controls all activity in cell • Surrounded by double membrane – nuclear envelope • Contains chromatin and nucleolus The nucleus and rough endoplasmic reticulum Chromatin Nucleus Nucleolus Two membranes of nuclear envelope Pore Rough endoplasmic reticulum Ribosomes • Location: In nucleus • Appearance: Spaghetti; long thin strands • Nickname: • Function: Genetic material of cell (Chromosomes and DNA) • Location: In the nucleus; is condensed chromatin • Appearance: Darkened spot; meatball • Nickname: • Function: Makes ribosomes • May have more than one in nucleus • Location: Throughout the cell; found outside of the nucleus – Plant cell - does not fill completely • Appearance: Jello, gel like • Nickname: • Function: Surrounds organelles; chemical reactions occur • Composition: –70% water –30% proteins, fats, carbohydrates, and nucleic acids • Found inside of the cell • Any part of the cell that has its own structure and function • Carries out life processes • Location: Found floating around cell or attached to ER • Appearance: Dots • Nickname: Assembly workers • Function: Makes protein Most common organelle in a cell Only organelle in prokaryote • Location: Outside of nuclear envelope • Appearance: Series of folded membranes • Nickname:ER; Transportation system of cell • Function: Connects organelles, important role in biosynthesis reactions •Rough ER – make more membrane, modify proteins •Smooth ER – synthesis of lipids, break down harmful substances, storage of calcium ions The nucleus and rough endoplasmic reticulum Chromatin Nucleus Nucleolus Two membranes of nuclear envelope Pore Rough endoplasmic reticulum Ribosomes Smooth and rough endoplasmic reticulum Smooth ER Rough ER Nuclear envelope Ribosomes Rough ER TEM 45,000 Smooth ER Synthesis and packaging of a secretory protein by the rough ER Transport vesicle buds off 4 Ribosome Secretory (glyco-) protein inside transport vesicle 3 Sugar chain 1 2 Glycoprotein Polypeptide Rough ER Connections among the organelles of the endomembrane system Rough ER Transport vesicle from ER to Golgi Transport vesicle from Golgi to plasma membrane Plasma membrane Nucleus Vacuole Lysosome Smooth ER Nuclear envelope Golgi apparatus • Location: Above nucleus • Appearance: Flattened curved sac • Nickname: Packaging and Shipping Department • Function: Finishes making a protein; modifies, sorts, and packages protein for secretion The Golgi apparatus “Receiving” side of Golgi apparatus Golgi apparatus Golgi apparatus New vesicle forming “Shipping” side of Golgi apparatus Transport vesicle from the Golgi TEM 130,000 Transport vesicle from ER • Location: Throughout the cell • Appearance: Lima Beans • Nickname: Power House of Cell • Function: Supplies energy; organic molecules are broken down here to release energy • Contains small amounts of DNA and RNA • Surrounded by a double membrane • The more energy the cell needs, the more mitochondria the cell will have The mitochondrion Mitochondrion Outer membrane Inner membrane Cristae Matrix TEM 44,880 Intermembrane space • Location: In a plant cell and algae • Appearance: Varies • Nickname: Carbohydrate producer • Function: Produces carbohydrate • Chloroplasts • Carotene • Chromoplasts • Leucoplasts The chloroplast Chloroplast Inner and outer membranes Granum Intermembrane space TEM 9,750 Stroma • Location: Found throughout cell; Most abundant in plants • Appearance: Circular; huge in plant in center of cell • Nickname: Storage room • Function: Stores water, nutrients, and wastes Central vacuole in a plant cell Nucleus Chloroplast Colorized TEM 8,700 Central vacuole Found in protists Spokes collect excess water from cell Hub expels it to the outside Contractile vacuoles in Paramecium Contractile vacuoles LM 650 Nucleus • Location: Throughout the cell; often attached to vacuoles in animal cell; more common in animal cells • Appearance: Circles • Nickname: Recycling Center • Function: Enzymes inside will break down large molecules or worn down molecules and make them available for reuse; digestion, fight off foreign pathogens • Those affected are missing one or more hydrolytic enzymes of the lysosome • Pompe’s Disease: Harmful amounts of polysaccharide glycogen accumulates in muscle and liver cells • Tay Sachs Disease: Lysosomes lack enzyme needed to break down a lipid abundant in nerve cell membranes Lysosome formation and functions Rough ER 1 Transport vesicle (containing inactive hydrolytic enzymes) Golgi apparatus Plasma membrane Engulfment of particle Lysosome engulfing damaged organelle 2 “Food” Lysosomes 3 Food vacuole 5 4 Digestion Lysosome formation QuickTime™ and a YUV420 codec decompressor are needed to see this picture. Lysosomes in a white blood cell Lysosome TEM 8,500 Nucleus Lysosome breaking down damaged organelles Lysosome containing two damaged organelles Peroxisome fragment TEM 42,500 Mitochondrion fragment • Location: Throughout the cell • Appearance: Strands or tubes • Nickname: Scaffolding of cell • Function: Gives the cell its shape, internal organization, and movement • Appearance: Solid and threadlike (made from protein – actin) • Function: Provides structural support; Assists in cell movement and shape change • Appearance: Hollow, flexible strands; Made of proteins (tubulin) • Function: Scaffolding used to construct the cell; Create pathways for the movement of organelles • Appearance: Ropelike structure; Made of fibrous proteins • Function: Reinforces cell’s shape and anchors organelles Fibers of the cytoskeleton Tubulin subunit Actin subunit Fibrous subunits 7 nm Microfilament 25 nm 10 nm Intermediate filament Microtubule • Microtubules wrapped in extension of cell membrane • Used for movement Structure of a eukaryotic flagellum or cilium Flagellum Electron micrographs of cross sections: Outer microtubule doublet TEM 206,500 Central microtubules Radial spoke Dynein arms Flagellum Basal body (structurally identical Basal body to centriole) TEM 206,500 Plasma membrane Colorized SEM 4,100 Cilia on cells lining the respiratory tract LM 600 Undulating flagellum on a sperm cell • Location: Above nucleus; only in animal cells • Appearance: Firewood; made up of microtubules • Nickname:Reproduction Center • Function: Important in cell reproduction • Location: Outside of cytoplasm; surrounds cell • Appearance: Phospholipid Bilayer; semifluid • Nickname:Cell Membrane; Fluid Mosaic • Function: Determine what can enter or leave the cell • Hydrophobic interior of the bilayer makes it selectively permeable –Nonpolar, hydrophobic molecules can pass through –Polar molecules and ions cannot Figure 5.10 Plasma membrane in cross section TEM 200,000X Outside of cell Cytoplasm Figure 5.11A Phospholipid molecule + CH3 CH2 N CH3 CH3 CH Hydrophilic head 2 Phosphate group O P O O– O CH2 CH CH2 O O C O O C CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH CH2 CH2 CH2 CH2 CH2 CH2 Symbol CH CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH2 CH3 CH3 Hydrophobic tails Figure 5.11B Phospholipid bilayer Water Hydrophilic heads Hydrophobic tails Water Figure 5.12 The plasma membrane and extracellular matrix of an animal cell Fibers of the extracellular matrix Carbohydrate (of glycoprotein) Glycoprotein Glycolipid Plasma membrane Phospholipid Proteins Microfilaments of cytoskeleton Cholesterol Cytoplasm • Location: Outside of cell membrane; only found in plants, bacteria, and fungi cells • Appearance: Made of stiff fibers of cellulose and other complex carbohydrates • 10 – 100 times thicker than cell membrane • Function: Provides support and protection to cell Does not determine what may enter or leave the cell •Often remains intact after the rest of the cell has died To function in a coordinated way as part of a tissue, the cells must have cell junctions Plasmodesmata: Channels between adjacent plant cells, form a circulatory and communication system connecting the cells Nuclear Membrane • • • • Nuclear envelop Double membrane perforated with pores Surrounds the Nucleus Controls the flow of materials into and out of nucleus •Cilia – short numerous appendages that propel organism •Flagella – longer, less numerous appendages Plasmodesmata • Channels between adjacent plant cells • Forms a circulatory and communication system connecting cells in plant tissues Plant cell walls and cell junctions Walls of two adjacent plant cells Vacuole Plasmodesmata Layers of one plant cell wall Cytoplasm Plasma membrane • Sticky layer of glycoproteins that animals secrete • Function: Helps holds cells together in tissues, protects, supports • Connects animal cells • Tight Junctions: bind cells very tightly, forms a leak proof sheet (digestive system) • Anchoring junctions: Forms strong sheets; rivet cells together with cytoskeleton (skin and heart muscle) • Gap Junctions: allow small particles to flow through (flow of ions in muscle contractions) • Very cool cell animations • http://www.youtube.com/watch?v=4AnPVuzF 7CA motor protein • http://www.youtube.com/watch?v=hOnMU2b AbJI&NR=1 The Harvard 3D Cell Animation • http://www.youtube.com/watch?v=fZZ3DD_t V9k Harvard Cell Animation • http://www.youtube.com/watch?v=5JjJDFwsl Eo&feature=fvw The Living Cell