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Lecture 2 – Tubular GI Histology of the Esophagus, Stomach and Intestines Dr. Pillinger Lecture on Friday, 8/3/2012 Lecture Material Only General Structure of the Digestive tract 1a. What is this layer 1b. What does this layer include (three things) 2. What is this layer 3a. What is this layer 3b. What does this layer include (two things) 4. What is this layer Components of Wall of GI Tract Meissner submucosal plexus 10 5 Auerbach myenteric nerve plexus 6 1 7 2 3 9 4 4 8 LP = lamina propria MM = muscularis mucosae MP = muscularis externa SM = submucosa: PG = parasympatheti c ganglion Components of Wall of GI Tract 1 CM = circular layer of smooth muscle 3 2 Auerbach’s myenteric plexus LM = longitudinal layer of smooth muscle Histology of Esophagus Squamous epithelium Lamina propria with papilla mucosa Muscularis mucosa Lymphoid follicle in submucosa submucosa Inner circular muscle Connective tissue Outer longitudinal muscle muscularis Histology of Esophagus Normal esophageal mucosa 1 Epithelium of Non-keratinizing stratified squamous mucosa 2 lamina propria coming into epithelium (papilla formation Esophagus (cross sections) 1 – name boxes Top to bottom 3? 7? 4? 5? 6? 2? Esophagus (Masson's trichrome) 8 7 2 6 5 1 4 3 CM = inner circular layer of muscularis propria E = epithelium G = seromucous gland LM = outer longitudinal layer of muscularis propria LP = lamina propria Ly = lymphoid aggregates MM = muscularis mucosae MP = muscularis propria SM = submucosa Type of epithelium? Squamous Type of epithelium? Columnar pylorus Normal GE junctions Rugae Semi-aligned drawing & micrograph of gastric glands Body of the stomach C = circular layer of muscularis externa GP = gastric pits L = longitudinal layer of muscularis externa M = mucosa MM = muscularis mucosa O = oblique layer of muscularis externa Base of gastric gland E = enteroendocrine cell Pc = chief cells PI = parietal cells Stomach: Pyloric area Immunostain for Gastrin MM = muscularis mucosa G = gastrin secreting enteroendocrin e cells P = gastric pits Gastroduodenal junction (pylorus) B = Brunner glands CM = circular layer of muscularis D = duodenum LM = longitudinal layer of muscularis PS = pyloric sphincter S = stomach Submucosal glands Muscularis mucosa submucosa Dilated blood vessel Lymphatic vessels Inner layer of circular muscularis Normal small bowel Outer layer of longitudinal muscularis Auerbach’s myenteric plexus would be here Monkey duodenum B = Brunner's submucosal glands CM = inner circular layer of muscularis LM = longitudinal layer of muscularis M = mucosa MM = muscularis mucosa SM = submucosa V = villi © Elsevier. Young et al. Wheater’s Functional Histology 5e – www.studentconsult.com Goblet cells Inflammatory Cells Crypts villi Nuclei Lamina propria Muscularis mucosa Normal small bowel: histology Submucosa Intestine:Crypts of Lieberkuhn G = goblet cell Mt = mitotic figure P = Paneth cell E = enteroendocrine cell What layer of the GI tract is this? Duodenum What layer of the GI tract is this? Ileum B = Brunner's glands CM = inner circular layer of muscularis LM = longitudinal layer of muscularis M = mucosa MM = muscularis mucosa PC = plicae circularis SM = submucosa Sr = serosa V = villi C = crypts of Lieberkuhn LP = lamina propria What is this? Intestinal villi & crypts B = Brunner's glands CM = inner circular layer of muscularis LM = longitudinal layer of muscularis M = mucosa MM = muscularis mucosa PC = plicae circularis SM = submucosa Sr = serosa V = villi C = crypts of Lieberkuhn LP = lamina propria C = capillary L = lacteal Ly = lymphocytes Mu = smooth muscle fiber Mitosis Intestinal villi (a) H&E (b) PAS/iron H/orange G (c) H&E Slide 24 Folding of small intestine Fold, plica circulares M = muscularis externa P = plica circulares S = serosa SM = submuscosa V = villi What area of the GI tract is this? Ileum with Peyer’s patches Normal appendix S = serosa M = mesentery F = follicle LP = lamina propria Colon submucosa fat CM = circular layer of muscularis LM = longitudinal layer of muscularis LA = lymphatic nodule Goblet cells Recto-anal junction J = line of Hilton RM = rectal mucosa SS = skin