Download Zebrafish Development

17 hours in the life of a zebrafish embryo
Karlstrom and Kane, 1996
Blastula Stages
1-cell
2-cell
4-cell
8-cell
32-cell
512-cell
1000-cell
sphere
dome
30% epiboly
Transcription
begins
4 2/3 hrs
Kimmel et al., 1995
Gastrula Stages
50%
germ ring
shield
75%
90%
bud
Kimmel et al., 1995
10 hr
Somitogenesis
3-somite
6-somite
18-somite
26-somite
22 hr
Kimmel et al., 1995
Zebrafish development
Karlstrom and Kane, 1996
Early development
?
Gastrulation
It is not birth, marriage or death, but gastrulation,
which is truly the most important event in your life
Lewis Wolpert
Three germ layers
Side view
Animal pole
Skin & nervous system
Muscle & cardiovascular
Gut and liver
Vegetal pole
top view
Gastrulation simplified
Cell movements during gastrulation
involution
Convergenceextension
epiboly
Involution
Mesoderm & endoderm inside
Ectoderm outside
Epiboly
Convergence-Extension
convergence
extension
Convergence-extension
Convergence-extension
Convergence-Extension
Marlow et al., 1998
Dorsal-Ventral
Side view
ventral
dorsal
ventral
dorsal
top view
Fate maps reflect movement
Dorsal-ventral patterning
Shield
(Organizer)
The Organizer
Shield
(Organizer)
Repeating the Spemann organizer experiment
Bmp promotes ventral fates
Bmp signals promote ventral fates (skin, ventral mesoderm)
and oppose dorsal fates (neural, dorsal mesoderm)
Organizer signals are BMP binding factors:
Noggin, Chordin
Mutants in the dorsal-ventral pathway
Snailhouse (snh)
= bmp7 mutant
Chordino (din)
= chordin mutant
Summary
Questions
• How are gradients established and
interpreted?
• How do cells form distinct compartments?
• How do groups of cells arrange into
tissues and organs?
Neural Tube closure
Forming tubes
Navis and Bagnat, 2015
References
Feldman B, Gates MA, Egan ES, Dougan ST, Rennebeck G, Sirotkin HI, Schier AF, Talbot WS. (1998). Zebrafish
organizer development and germ-layer formation require nodal-related signals. Nature. 395:181-5.
Karlstrom RO, Kane DA. (1996). A flipbook of zebrafish embryogenesis. Development. 123:461.
Keller PJ, Schmidt AD, Wittbrodt J, Stelzer EH. (2008). Reconstruction of zebrafish early embryonic development by
scanned light sheet microscopy. Science. 322:1065-9.
Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. (1995). Stages of embryonic development of the
zebrafish. Dev Dyn. 203:253-310.
Marlow F, Zwartkruis F, Malicki J, Neuhauss SC, Abbas L, Weaver M, Driever W, Solnica-Krezel L. (1998). Functional
interactions of genes mediating convergent extension, knypek and trilobite, during the partitioning of the eye primordium
in zebrafish. Dev Biol. 203:382-99.
Navis A, Bagnat M 2015 Developing pressures: fluid forces driving morphogenesis. Current Opinion in Genetics &
Development 2015, 32:24–30