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-Unclassified/For Official Use Only- Anthrax Case Study: Amerithrax Investigation William So, MS, PhD Policy & Program Specialist FBIHQ/WMD Directorate Biological Countermeasures Unit Washington, DC, U.S.A. 011-202-324-5536 [email protected] Federal Bureau of Investigation -Unclassified/For Official Use Only- Purpose of Briefing • To provide the scope of the investigation undertaken and lessons learned regarding pathogen security (“suspicious” behavior indicators) Federal Bureau of Investigation -Unclassified/For Official Use Only- Background • September and October 2001 – Five letters (at least) containing Bacillus anthracis spores were sent through the United States Postal Service • Letters were sent to medial organizations (9/18/01) – New York Post (New York City, NY) – National Broadcast Company (NBC) (New York City, NY) – American Media, Inc. (AMI) (Boca Raton, FL) » Letter to AMI has not been recovered • Two letters were sent to U.S. Senators Leahy and Daschle (both letters recovered) (10/3/01) Federal Bureau of Investigation -Unclassified/For Official Use Only- Amerithrax Federal Bureau of Investigation -Unclassified/For Official Use Only- Victims • 22 individuals were confirmed with anthrax infections – 11 had cutaneous anthrax – 11 had inhalation anthrax • Five deaths (inhalation anthrax) • 31 others tested positive for exposure, but no infection • 10,000 were deemed at risk Federal Bureau of Investigation -Unclassified/For Official Use Only- Contamination • 35 postal facilities and commercial mailrooms – Trenton, New Jersey and Washington, DC facilities were most contaminated • Over 1.8 million pieces of mail were quarantined • 26 buildings near the U.S. Capitol – $27 million USD for cleanup Federal Bureau of Investigation -Unclassified/For Official Use Only- Scope of Investigation • • • • Task Force: FBI and USPIS 7 years 600,000+ investigator work hours 10,000+ interviews conducted (in 6 continents) • 80 searches • 6,000+ pieces of evidence • 5,730 environmental samples from 60 locations Federal Bureau of Investigation -Unclassified/For Official Use Only- Scope (continue) • 1,000+ potential suspects – International and U.S. • Assistance and cooperation from 29 governments, universities, and commercial laboratories • New forensic techniques required – Genetic laboratory tests developed and validated – Traditional forensic examination on biologically contaminated evidence Federal Bureau of Investigation -Unclassified/For Official Use Only- Suspect Profile • Scientific ability • Laboratory access to the Ames strain • Proximity to Princeton, New Jersey (where the letters were mailed) • Suspicious behavior • Tips from public and scientific community • Possible motivation/incentive Federal Bureau of Investigation -Unclassified/For Official Use Only- Bacillus anthracis - Ames • Ames strain of Bacillus anthracis – First isolated in Sarita, Texas in 1981 – Sample were sent to United States Department of Agriculture laboratory in Ames, Iowa • Hence the Ames strain • Only in 15 U.S. and 3 international laboratories Federal Bureau of Investigation -Unclassified/For Official Use Only- Mutations • Spores from letters were cultured – Generally incubated for 24-36 hours only – Colonies generally round, smooth edges • When cultures were incubated longer – Colony morphology differed (the edges of the colonies were not smooth) – Mutations in the genetic makeup caused these differences in morphology Federal Bureau of Investigation -Unclassified/For Official Use Only- FBI Repository (FBIR) • 1070 samples submitted • Genetic tests (forensic microbiology) were validated in 2007 • Based on the new genetic tests, it was determined that 8 samples were matches to those recovered from the letters • All of those samples were submitted from United States Army Medical Research Institute of Infectious Disease (USAMRIID) – RMR-1029 Federal Bureau of Investigation -Unclassified/For Official Use Only- Suspicious Indicators: Activities • Laboratory activities at USAMRIID – Sept 11-18, 2001 (9/18/01) – October 1-8, 2001 (10/3/01) • Laboratory access logs showed – Dr. Ivins spent long nights and weekends in the laboratory prior to the postmark dates – No such activities were noted in the months/years prior or after Federal Bureau of Investigation -Unclassified/For Official Use Only- Suspicious Indicators: Mental Health • Periods of severe depression/paranoia – Medication and counseling • Troubled marriage/home life and feeling of abandonment (close co-workers leaving/left) • Distant from co-workers except for two • Dislike for New York City • Complaint letter sent to politicians and media in the past • Research and funding in jeopardy Federal Bureau of Investigation -Unclassified/For Official Use Only- Suspicious Indicators: Lying/Deception • To investigators – Downgraded his expertise/experience (lyophilizer) – Put blame on co-workers – Submitted different samples than what was asked for to FBIR • Other deception – Scenario sent to CDC was improbable • Mr. Robert Stevens was diagnosed with inhalation anthrax • Dr. Ivins scenario indicated drinking infected water – E-mail tracking software – Habit of using false names, driving long distance to mail items Federal Bureau of Investigation -Unclassified/For Official Use Only- “I just heard that the Bin Laden terrorists for sure have anthrax and sarin gas” “Osama Bin Laden has just decreed death to all Jews and all Americans.” Federal Bureau of Investigation -Unclassified/For Official Use Only- Federal Bureau of Investigation -Unclassified/For Official Use Only- Short Biology Lesson Transcription DNA Translation RNA proteins Federal Bureau of Investigation -Unclassified/For Official Use Only- Federal Bureau of Investigation -Unclassified/For Official Use Only- Hidden Message • Dr. Ivins was fascinated with codes and puzzles – Books – Word puzzles sent to co-workers • Two potential hidden messages – “FNY”, a verbal assault on New York – “PAT”, a nickname for one of his former coworkers Federal Bureau of Investigation -Unclassified/For Official Use Only- Summary • Opportunity (access) • Motive (research in jeopardy) • Mental health issues • Proximity to envelopes • Language similarity • Guilty conscience • History of disguise/false identity • Unusual habit of mailing items from a distance • Inability to explain behavior Dr. Ivins committed suicide in Summer of 2008. Federal Bureau of Investigation -Unclassified/For Official Use Only- QUESTIONS William So, MS, PhD FBIHQ/WMD Directorate Washington, DC, U.S.A. 011-202-324-5536 [email protected] Federal Bureau of Investigation