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Investigation of antibacterial effects of Zizyphus jujuba P-02.08.5-022 H.I. OzkanI, Y. AticiII, T. YavuzIII, Y. ÖzelIV, A.A. HismiogullariI, Ö. YavuzI IBalikesir University, Faculty of Medicine, Department of Medical Biochemistry, Balikesir, Turkey, IIBalikesir University, Health Sciences Institute, Department of Veterinary Biochemistry, Balikesir, Turkey, IIIBalikesir University, Faculty of Medicine, Department of Medical Microbiology, Balikesir, Turkey, IVBalikesir University, Health Sciences Institute, Department of Medical Microbiology, Balikesir, Turkey Plants are most important parts of natural resources that alternatively referred to synthetic drugs for reasons such as being less side effects and lower costs. Ziziphus jujuba Miller (Z. jujuba), a plant used in traditional medicine, is one of the most important Ziziphus species belonging to Rhamnacea family. The fruit and seeds of this plant are used different purposes such as anti-inflammatory, antioxidant, immune-stimulant and wound healer. In this study, we investigated the antibacterial effects of Z. jujuba. The aims of this study were to screen the antibacterial activity of Z. jujuba. The extract was obtained from Z. jujuba fruits pulverized with the aid of ball mill using 50% aqueous-ethanol solution. Extracts were screened for antimicrobial activity against six different standard strains of bacteria by determining minimum inhibitory concentration (MIC) according to CLSI criteria. Serial dilutions are made between 64 mg/mL and 0.031 mg/mL concentration range. The lowest concentration of wells that no visible growth has been accepted as MIC value. Materials in the MIC and lower concentrated wells were transferred to 5% Sheep Blood Agar petri dishes for calculation of minimal bactericidal concentration (MBC). The lowest concentration that no colony formation has been accepted as MBC value. jujuba showed the most potent effect on strain of S. aureus ATCC 29213 is Grampositive cocci (MIC: 2 mg/mL). The MIC values of other Gram-positive bacteria S. aureus ATCC 43300, E. faecalis ATCC 51219, L. monocytogenes F 1483 and M. smegmatis CMM 2067 were detected as 8, 16, 8 and 8 mg/mL respectively. MIC values of Gramnegative bacilli were detected as > 64 mg/mL. Consequently, Z. jujuba was found to be effective on Gram-positive cocci bacteria ( S. aureus ATCC 29213, S. aureus ATCC 43300 and E. faecalis ATCC 51219). The strongest effect was observed on S. aureus ATCC 29213 strain. In contrast, extract showed less effect on Gram-negative bacilli.