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Antibiotic resistance of bacteria isolated from mice’s intestine in Lahijan
Zhaleh Dezfoolimanesh1 *, MohammadRasol Tohidnia2, Fatemeh Darabi3, Mojdeh Assarezadegan4
1. Dept. of Microbiology, Paramedicschool, kermanshah University of Medcial Sciences, Kermanshah, Iran
2. Dept. of Radiology, Paramedicschool, kermanshah University of Medcial Sciences, Kermanshah, Iran
3. Dept. of Anestesiology, Paramedicschool, kermanshah University of Medcial Sciences, Kermanshah, Iran
4. Dept. of Paramedicschool, kermanshah University of Medcial Sciences, Kermanshah, Iran
Received: 14 March, 2009
Accepted: 4 August, 2009
Abstract
Background: Disease transmission by rats and mice is a worldwide problem of big cities,
which is important economically and also a significant health problem. This study was carried
out to identify bacterial species find out antibiotic resistance of isolated bacteria in local rat's
population in Lahijan - Iran.
Methods: Cluster random sampling design was used to study a total sample of 100 rodent
species, from 13 different regions within the Lahijan city. Rats were killed by using ether
inhalation and sterile swabs were used to collect samples from cecum.
The collected swabs were cultured for bacterial growth on different media. Cold enrichment
phosphate buffer was used for isolation of Yersinia Enterocolitica . Antibiotic sensitivity was
determined by using Kirby-Bauer's method. SPSS software was used for data analysis.
Results: A total of 100 rats were entrapped. 94 of them were of Rattus norvegicus and the
other 6 of Rattus rattus types. The results showed that 70% of the rodents were carriers of
proteus species, 34% klebsiella, 20% Entero pathogenic Ecoli, 4% salmonella typhimurium,
45% enterobacter cloacae, 64% citrobacter spp, 75% non-pathogenic E coli, 19% serratia
marscesins, 41% hafnia alveoi, 57% pseudomonas aeroginossa, 10% yersinia enterocolitica,
31% aureus, 38% coagulase negative staphylococcus, 13% streptococcus faecalis and 40%
bacillus cereus. The most sensitive antibiotics included ciprofloxacin, nalidixic acid and
cotrimoxazole.
Conclusion: We concluded that mice and rats are potentially able to transmit diseases to
human. Further studies about health and economic effects of rats in large cities are
recommended.
Keywords: Bacterial enteropathogens, Rattus rattus, Rattus norvegicus, Bacterial resistance
Behbood, (2009), 13(3): 242-251
Corresponding author’s contact: Paramedicschool, kermanshah University of Medcial Sciences, Kermanshah, Iran
*
Tel: +98 831 8279697, Email: [email protected]
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