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The Guppy Project This project gives students a hands-on approach to learning heredity and how traits are passed from generation to generation. Curriculum/State Standard Camden Diocese Science Curriculum – Life Science Content E:1,2,4 Mendel’s Theory of Genetics, Chromosomes and genes, dominance/recessive, genetic engineering New Jersey Core Curriculum Content Standards – Standard 5.2.15, Standards 5.7.3,5,,9,13 Overview Students set-up 3 breeding trios of guppies, each trio having different traits. After allowing guppies to reproduce and giving time for maturity, students determined male and female guppies, separated them and observed differing characteristics between individuals and the sexes. Through selected breeding, new breeders were established and allowed to breed based on their inherited traits. These traits will be observed for the purpose of learning how these new traits, when combined, are passed from parent to offspring. Objectives • The student will apply Mendelian genetic principles in a “real-life” situation. • The student will, through direct observations, determine recessive, dominant, and sexlinked traits in guppies. 8-12 GRADE LEVEL • The student will be able to compile data gathered through the project to make a clear determination of the outcome of the selected characteristics that were being studied Materials 6 – 10 gallon fish tanks, cart 6' x 3' ( I used stainless steel and welded the cart together), pump, heater, 1 – 30 gallon trash can, filter system, air system, 30 ft. PVC 1/2" pipe, 12 – 1/2" PVC elbows, 200 ft 1/4" air tubing, 2 – 5 way gang valves, 3 – 2 quart pitchers, 3 PVC Tees, additional tubing for pump and filter system (depending on what you use), fish, additional support information on guppies. Please note that additional supplies may be needed to start your project. Readiness Activity Students spent two days learning about the history, anatomy, mating practices and breeding/birthing cycles of guppies. They spent a third day doing preliminary observation on the original parents used. 1 YEAR Continued on the back . . . $1,000 TOTAL BUDGET THIS WINNING LESSON PLAN WAS SUBMITTED BY: Lawrence R. Young Sacred Heart Grammar School 922 East Landis Avenue, Vineland, NJ 08360 “The Guppy Project” project continued . . . Strategies/Activities Once the project is set-up, label each of the six tanks, using numbers 1-6. Acquire your parent trios. This can be done through your local pet store or from an actual breeder. I suggest obtaining the guppies from a breeder, this allows you to know that the strains are pure and not mixed. The local community college in my area supplied our guppies from their aquaculture program. Separate each trio (consisting of one male and two females) into separate tanks. Each trio should consist of males and females from the same strain. After the students have received the preliminary information, allow them to observe the breeding trios for characteristics that are being studied. Characteristics to be studied are size, scale color and pattern, caudal tail shape, and dorsal fin size and shape. These are only a few of the possible characteristics that can be studied. I require each student to obtain a separate notebook for the project to log all data, drawings, and other information given. The students should keep accurate written and drawn descriptions of the observations. Assuming that the females are not already pregnant by the male, the first drop should occur around a month to a month and a half after the set-up. If the females are already pregnant from the trio male, keep watch over the females. A tip on when to expect the delivery of the drop: you will be able to see, very clearly, the eyes of the young frey (new-born guppies). From this point you have about a day. Birth generally occurs early morning to late morning. You will need to separate the frey immediately, because the parent guppies will eat them as they are born. To separate the frey, simply place a divider in the tank and place the frey on the unoccupied side of the tank. Delivery may take up to four or five hours and usually will yield between 19 and 30 offspring. Once the new frey are all delivered, allow the students to make their next set of observations. They should include number of offspring born, written descriptions and drawings of at least four or five of them, including size, shape, and color. In about 4-5 weeks the students will make their next set of observations. At this time the male’s reproductive structure, called the gonopodium is visible. Other traits are starting to appear as well, including color. Allow students to write and draw the differences between males and females. It is important that you separate the males and females as the students identify the differences. Generally, male and females become reproductively active at this time and to control the genetics and breeding cycles males and females need to be separated. I have used tanks 5 and 6 as my male and female tanks. This allows the fish to mature and gain all of their characteristics, but eliminates the possibility of mating before the students know the characteristics. By week 10, the offspring should gain their full characteristics and the students need to make another set of observations for both male and females. After the observation, the students have the task of determining which characteristics are dominant, recessive, and sexlinked. This may take a few breeding cycles to fully gain an accurate conclusion. The students can then determine, of the offspring, which contain suitable breeding characteristics and which do not. The guppies that do contain the suitable characteristics are placed in tank 6 and the others are sold off at the end of the semester or year during a fish sale. The profits from the sale are then used to maintain any replacements of parts or other maintenance items that may arise, creating a self-supporting program. Once the guppies are separated and breeders have been established, run a few more breeding cycles, and then you can have the students start to mix the strains to develop new strains based on the desired characteristics. The students will have to apply the knowledge gained from previous years to determine if the cross will work and yield the proper outcomes. Properly developing a new strain will take a year or two, so be patient and allow the students to take ownership of the project. Other considerations to allow this to be the students’ project are train six or seven students to perform water quality tests once a week. My students test for oxygen, pH, nitrates, nitrites and ammonia. Allow them to carefully feed the guppies and siphon the tanks but use discretion. Culminating Activity Students are assigned a final report that detail the project and their findings. Results, conclusions, drawings, data collected, and suggestions for the next class of students are be included in the report. Students also attend Cumberland County College to learn more about Aquaculture. Activities include chemical testing of water and fish weighing. Evaluation Students are evaluated through direct teacher observations during lab times, their Guppy Project Notebook, quizzes, and a final report.