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ABBREVIATIONS
Ab
antibody
IHHN(V)
ABTS
2,2’-azino-di-(3-ethyl-benzthiazoline)6-sulphonic acid
infectious hypodermal and haematopoietic
necrosis (virus)
IHN(V)
infectious haematopoietic necrosis (virus)
Ag
antigen
IMN(V)
infectious myonecrosis (virus)
AS
Atlantic salmon (cell line)
IPN(V)
infectious pancreatic necrosis (virus)
ASK
Atlantic salmon kidney (cell line)
ISA(V)
infectious salmon anaemia (virus)
BCIP
5-bromo-4-chloro-3-indoyl phosphate
ISH
In-situ hybridisation
BF-2
bluegill fry (cell line)
ITS
internal transcribed spacer
BKD
bacterial kidney disease
KF-1
koi fin (cell line)
BP
Baculovirus penaei
LOS
lymphoid organ spheroids
BSS
balanced salt solution
LOV
lymphoid organ virus
CCB
Cyprinus carpio brain (cell line)
MAb
Monoclonal antibody
CCO
channel catfish ovary (cell line)
MDT
Mean death time
CCV(D)
channel catfish virus (disease)
MEM
Minimal essential medium
CHSE-24
chinook salmon embryo (cell line)
MHC
Major histocompatibility complex
CIA
Cowdry type A inclusion bodies
MLV
Modified live virus (vaccine)
CPE
Cytopathic effect
m.o.i.
multiplicity of infection
DEPC
Diethyl pyrocarbonate
MSV
Master seed virus
DIG
digoxigenin
NI
Neutralisation index
DNA
deoxyribonucleic acid
OGP
1-octyl-beta-D-glucopyranoside (buffer)
dNTP
deoxynucleotide triphosphate
OPD
Orthophenyldiamine (chromogen)
ECV
European catfish virus
OPG
EDTA
Ethylene diamine tetra-acetic acid
Oxalase-phenol-glycerin (preservative
solution)
EHN(V)
epizootic haematopoietic necrosis (virus)
ORF
Open reading frame
ELISA
enzyme-linked immunosorbent assay
PAGE
Polyacrylamide gel electrophoresis
EPC
epithelioma papulosum cyprini (cell line)
PAP
ESV
European sheatfish virus
Peroxidase-antiperoxidase (staining
procedure)
EUS
epizootic ulcerative syndrome
PAS
Periodic acid-Schiff (reaction)
FAT
Fluorescent antibody test
PBS
Phosphate buffered saline
FBS
fetal bovine serum
PCR
Polymerase chain reaction
FCS
fetal calf serum
FEV
fish encephalitis virus
FHM
fathead minnow (cell line)
FITC
fluorescein isothiocyanate
GAV
gill-associated virus
GF
grunt fin (cell line)
H&E
haematoxylin and eosin
PD
Protective dose
PFGE
Pulsed field gel electrophoresis
PFU
Plaque-forming unit
PHA
Passive haemagglutination (test)
PPD
Purified protein derivative
PPLO
Pleuropneumonia-like organisms
PRN
Plaque reduction neutralisation
PSG
Phosphate buffered saline glucose
RBC
Red blood cell
RFLP
Restriction fragment length polymorphism
HBSS
Hanks’ balanced salt solution
HEPES
N-2-hydroxyethylpiperazine,
N-2-ethanesulphonic acid (buffer)
HP
hepatopancreas
RK
Rabbit kidney
HRPO
Horseradish peroxidase
RPM
Revolutions per minute
IF
immunofluorescence
RSA
Rapid serum agglutination
IFAT
Indirect fluorescent antibody test
RT-PCR
Ig
immunoglobulin
Reverse-transcription polymerase chain
reaction
SAT
Serum agglutination test
2016 © OIE - Manual of Diagnostic Tests for Aquatic Animals - 16/08/2016
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Abbreviations
SDS
Sodium dodecyl sulphate
TSI
Triple sugar iron (medium)
SPF
Specific pathogen free
VB
Veronal buffer
SPG
Sucrose phosphate glutamic acid
VBS
Veronal buffered saline
SRBC
Sheep red blood cells
Vero
African green monkey kidney (cells)
TCID50
Median tissue culture infective dose
VN
Virus neutralisation
TMB
Tetramethyl benzidine
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2016 © OIE - Manual of Diagnostic Tests for Aquatic Animals - 16/08/2016