Download BD FACSuite Software Quick Reference Guide for

BD FACSuite Software Quick Reference Guide
for the Experiment Workflow
This guide contains instructions for using BD FACSuite™ software with the BD FACSVerse™ flow cytometer using
the experiment workflow. Data can be acquired and analyzed in an experiment or in a worklist, as shown in the
following diagram.
Worklist
Analyze
Data
Create experiment
from an assay
t
Acquire
Data
t
t
Acquire
Data
Save experiment
as an assay t
Perform setup
& QC
t
Start up
system
t
t
Experiment
t
Workflow Overview
Shut Down
System
Analyze
Data
Before starting your daily workflow, ensure that your lab’s software
administrator has performed all the initial setup tasks as specified in
the BD FACSVerse System Reference.
Start up the system
1
Log in to BD FACSuite software.
2
Check the fluid levels.
3
Check the connection and fluidics status.
4
Verify that the 20-minute laser warmup has been completed.
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Performance QC and Assay Setup
Performance QC
1
Open the Setup & QC workspace.
Verify the bead
lot ID.
Select Setup & QC.
Click Start.
2
Run the BD FACSuite™ CS&T Research beads.
3
View the Setup and QC Report and QC Tracking tabs.
Verify that the Performance QC passed.
Assay and Tube Settings Setup
1
Select the Setup & QC tab.
Select the Assay & Tube Settings Setup task.
Click Select.
2
Select the Tube Settings tab.
Select the additional
tube settings that you
want to set up.
3
Run the BD FACSuite CS&T Research beads.
Verify the Lot ID.
Click Start.
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Acquire Data
1
Open the Experiment workspace.
Select File > Rename
to rename the experiment.
Select Acquire Data
In Experiment.
2
Create plots, gates, statistics, and a hierarchy needed for acquiring data.
Use the Worksheets toolbar to create
plots, gates, statistics, and a hierarchy.
Right-click a plot and select
Properties to modify it.
3
Install a sample tube onto the cytometer. Click Preview.
Verify that the voltage
and threshold settings in
the default tube settings
are appropriate.
Click PMTV to enable the
sliders in the worksheet
to make adjustments
to the PMT voltages,
as needed.
Or, apply the appropriate
tube settings.
See Step 5.
Verify that the gates
and populations are
set appropriately.
4
Click
Stop.
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5
Double-click the tube to modify the tube properties.
Rename the tube.
Click Select to apply different tube
settings as needed.
Add or remove fluorochromes
as needed.
Change
fluorochromes
as needed.
Clear any A, H, or
W measurements
not needed.
Specify reagent labels, keywords,
and acquisition criteria as needed.
6
Click
Next in the Data Sources panel to create additional tubes, as required.
7
Click
Acquire.
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Analyze Data
1
Create any additional plots, gates, and statistics in the worksheet, or create a report.
Customize plots
by right-clicking a
plot and selecting
Properties.
2
Perform quality control of the analysis.
Verify that
gates are set
appropriately
for all samples.
Use the population
hierarchy to verify
parent/child
relationships.
3
Do one of the following to print or export the results.
• Select File > Print to print the active worksheet.
• Select File > Export to PDF to create a PDF of the active worksheet.
Shut Down the System
1
Select
Cytometer > Daily Clean.
2
Select
Cytometer > Shutdown.
3
Log out of the software, if necessary.
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Additional Information
Any experiment can be used to create a user-defined assay and used in a worklist to acquire and analyze data.
Additionally, experiments can be created from assays and used to acquire and analyze data in the Experiment workspace
or saved as an assay to be used in a worklist.
Creating an Assay from an Experiment
1
Open an existing experiment or create a new one.
2
Select
File > Create Assay.
Enter a name.
Select a report to display,
if needed.
Click OK.
Creating an Experiment from an Assay
1
Select
File > New Experiment from Assay.
2
Modify the assay as needed.
3
Select
File > Create Assay to save the experiment as an assay and acquire data in a worklist.
Alternatively, you can continue with data acquisition and analysis in the Experiment workspace.
Class 1 Laser Product.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures.
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