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Biofilms 4 Programme Biofilms 4 4th International Conference Final Programme Microbial communities in disease Wednesday, 1st September 09:00 – 13:00 Registration 12:00 – 13:30 Buffet lunch 13:30 Welcome, followed by The Integration of the Biofilm Concept into the Diagnosis and Treatment of Musculoskeletal Infections Keynote Speaker: Bill Costerton – Allegheny-Singer Research Institute, USA 14:10 Session 1: Microbial communities in disease Biofilms and chronic infections Chair and Invited Speaker: Thomas Bjarnsholt, University of Copenhagen, Denmark 14:30 Differential response of human keratinocyes to diffusible products from planktonic and biofilm cultures of Staphylococcus aureus Garth James, Montana State University, USA 14:50 Polymicrobial, pathogenic biofilms in pediatric adenoids: a cultureindependent approach to diagnosing biofilm infections Luanne Hall-Stoodley, University of Southampton Wellcome Trust CRF, UK 15:10 The Influence of Selected Autochthonous Bacteria on the Establishment of Methicillin Resistant Staphylococcus aureus and Pseudomonas aeruginosa in a Novel Wound Simulator Angela Oates, University of Manchester, UK 15:30 Characterization of biofilms on hemodiaysis catheters Steve Jones, Division of Nephrology, University of Calgary, Canada Surface engineering and biofilm tribology 15:30 – 16:00 Coffee break 16:00 Session 2: Surface engineering and biofilm tribology Antimicrobial effect of polymer brush coatings on staphylococcal biofilms Chair and Invited Speaker: Henny van der Mei, University of Groningen, The Netherlands 16:20 From nanotechnology to microbiology - antibiofilm activity of nanosized magnesium fluoride Ehud Banin, Bar-Ilan University, Israel 16:40 Antibiofilm coatings developed from a bio-inspired approach Thomas Blin, University of Rouen, France 17:00 Tribological behaviour of oral mixed biofilms Helena Cruz, DEB - University of Minho, Portugal 17:20 – 19:00 Welcome Drinks and Poster Session 1 Biofilms 4 Programme Thursday, 2nd September Global scale biofilm systems 08:30 09:00 Session 3: Global scale biofilm systems Mitigation and mediators of global change Chair and Invited Speaker: Hilary Lappin-Scott, Swansea University, UK 09:20 Utility of Biofilms in Geologic Carbon Sequestration Robin Gerlach, Montana State University, USA 09:40 Pathogen interactions within phylloplane biofilms: importance of the VBNC state Bill Keevil, University of Southampton, UK 10:00 Biophysical basis for the geometry of coniform biofilms Alexander Petroff, Massachusetts Institute of Technology, USA 10:20 – 10:50 Community ecology and evolution 10:50 Coffee break Session 4: Community ecology and evolution Influences on the composition and stability of microbiome communities during development Chair and Invited Speaker: Andrew McBain, University of Manchester, UK 11:10 Mutability of polyspecies bacterial biofilm community members Larisa Magdanova, Institute of Ecology and Genetics of Microorganisms, Russian Federation 11:30 The significance of oxygen gradients on the evolution of biofilm formation by Pseudomonas fluorescens SBW25 in static liquid microcosms. Anna Koza, The SIMBIOS Centre, University of Abertay Dundee, UK 11:50 Quantification of food web interactions on semi-natural biofilms. Martina Erken, Helmholtz Centre for Environmental Research, Germany 12:10 – 13:40 13:40 Novel biotechnology and bioengineering Mechanisms and consequences of biofilm dispersal Keynote Speaker: Staffan Kjelleberg – University of New South Wales, Australia Lunch break Session 5: Novel biotechnology and bioengineering Mass-spring models for individual-based microbial interactions and biofilm formation Chair and Invited Speaker: Cristian Picioreanu, Technical University of Delft, The Netherlands 14:00 Study of antibiotics diffusion-reaction in biofilms by dynamical fluorescence (FLIM,FCS) Karine Steenkeste, ISMO, France 14:20 Transcriptomic studies of putative gene clusters involved in biofilm formation & EPS biosynthesis of Acidithiobacillus ferrooxidans Söeren Bellenberg, University of Duisburg-Essen, Germany 14:40 Effects of different acidophilic, moderately thermophilic microorganisms on attachment and biofilm formation on pyrite 2 Biofilms 4 Programme Nanni Noel, University of Duisburg-Essen, Germany 15:00 – 15:30 Structural dynamics and emergent properties of biofilms 15:30 Coffee break Session 6: Structural dynamics and emergent properties of biofilms Biofilm matrix functionality Chair and Invited Speaker: Thomas Neu, Helmholtz Centre for Environmental Research 15:50 Biofilm physical properties from the inside Nelly Henry, Institute Curie, France 16:10 Bacillus subtilis biofilms on immersed surfaces: there is a life below the pellicle Romain Briandet, INRA, France 16:30 Role of eDNA in biofilms of Shewanella oneidensis MR-1 Kai Thormann 16:50 – 18:00 19:30 Poster session Conference dinner – Winchester Cathedral Poster prize announcement Modulation of biofilm communities Friday, 3rd September 09:10 09:30 09:50 10:10 Signalling and communication in biofilms 10:30 – 11:00 11:00 Session 7: Modulation of biofilm communities The driving and shaping of biofilm functions by protozoan predation Chair and Invited Speaker: Carsten Matz, Helmholtz Centre for Infection Research, Germany Identification of Small Molecule Inhibitors of Biofilm Formation by Salmonella Typhimurium and Pseudomonsa aeruginosa and Investigation of Their Mode of Action Hans Steenackers, Catholic University of Leuven, Belgium Environmental triggers and intracellular signaling of biofilm dispersal Nicolas Barraud, University of New South Wales, Australia Analysis of Multiple Hypotheses for Antibiotic Tolerance of Staphylococcus epidermidis in Biofilms Phil Stewart, Montana State University, USA Coffee break Session 8: Signalling and communication in biofilms Autoinducer-2: Role in Inter-species Interactions between Bacteria Indigenous to the Human Microbiome Chair and Invited Speaker: Alex Rickard, University of Michigan, USA 11:20 A Novel Cell-To-Cell Communication Molecule, cis-2-Decenoic Acid, Acts To Induce Bacteria To Transition From A Biofilm Lifestyle To An Active Disseminating Lifestyle David Davies, Binghamton University, USA 11:50 Remote control of antibiotic resistance within bacterial communities Jean-Marc Ghigo, Institut Pasteur, France 12:10 Denitrification and nitric oxide production in laboratory, in vivo and ex vivo biofilms of the oral cavity and nasopharynx. Paul Stoodley, University of Southampton, UK 3 Biofilms 4 Programme 12:30 – 14:00 Biofiilm development: a multidisciplinary approach 14.00 Lunch break Session 9. Biofilm development: A multidisciplinary approach The role of the Pel polysaccharide in Pseudomonas aeruginosa biofilm development Chair and Invited Speaker: Matt Parsek, University of Washington, USA 14:20 dATP/ATP in biofilm formation and pathogenesis Chuanwu Xi, University of Michigan, USA 14:40 Regulatory systems required for biofilm development and the maintenance of the planktonic mode of growth Karin Sauer, Binghamton University, USA 15:00 Growth and development of 3-dimensional structures in biofilms and cancer - an integrated approach Samuel Collins, University of Southampton, UK 15:20 Long-term evolutionary dynamics of Pseudomonas aeruginosa in a human host environment Keynote Speaker: Soeren Molin – Technical University of Denmark 15:50 Closing comments, departure Biofilms 4 4th International Conference 4