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Name _____________________________ Period ____Date _________________ Squid Dissection Background information: Squid are cephalopods which is Greek for ‘head foot’. They have a mantle with 2 fins, 8 arms and 2 tentacles (used for grabbing prey). They move by jet propulsion, allowing water into their mantle and forcing it out through their siphon. They use their siphon and fins to make quick changes in direction. They have three hearts: one for their body systems and two gill hearts; one located at the base of each gill pair so that they can pump oxygen to their muscles quickly, thus enabling them to swim fast. They feed at night in schools near the surface, eating plankton, fish, other squid, and crabs. Their predators are penguins, other squid, eels, toothed whales, seals, jellyfish, fish, and humans. Giant squid live very deep in the ocean and are eaten by sperm whales. For defense, they can squirt out a cloud of ink, change color to match their surroundings, make a jet propelled escape, maneuver quickly, or bite with their beak. Materials: 1 squid per group scissors water and paper towels for clean up 1 dissecting tray per squid 1 dissecting probe 1 Procedure: External Anatomy: 1. Locate the siphon. The siphon is found on the ventral side of the squid between the two eyes. 2. The tentacles and arms are attached to the head of the squid. Locate and compare the two. Examine the suckers on the end of the tentacles. 3. Find the two large eyes on the head of the squid. 4. Locate the body, which is covered by the mantle, and fins. The body is covered by tiny spots known as chromatophores that can change color & allow the squid to camouflage itself. 5. Spread apart the tentacles and use a probe to locate the mouth at the base of the tentacles. The beak can be felt inside the mouth and is a dark color. Label the following parts on the squid diagram below. (siphon, tentacles, arms, eye, mantle, fins, beak & head) 2 Questions 1. How many arms does the squid have? How many tentacles? _________________________________________________________________ _________________________________________________________________ 2. Based on the structure of the arms and the tentacles, describe how their purposes might differ. What do the arms do and what do the tentacles do? _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 3. What is the function of the siphon? _________________________________________________________________ _________________________________________________________________ 4. If water shoots out the siphon to propel the squid in the water, which direction does the squid swim; head-first, or tentacles-first? _________________________________________________________________ _________________________________________________________________ 5. Name two features that are adaptations for the squid’s predatory life. _________________________________________________________________ _________________________________________________________________ 6. Name two traits that the squid shares with other mollusks. ________________________________________________________________________ ________________________________________________________________________ 7. What specialized cells allow squid to blend in with their surroundings? _________________________________________________________________ 3 Procedure: Internal Anatomy 1. Turn the squid ventral side up. Lift the mantle up above where the siphon is; it should be loose and easy to pull up. Use scissors to make one long incision from the bottom of the mantle, above the siphon, to the tip of the mantle next to the fins. Open the mantle to expose the structures inside. 2. Find the esophagus, this is best found by looking into the mouth and seeing where it leads, use a probe to carefully poke into the mouth. 3. To find the stomach, follow the esophagus toward the posterior end of the squid. 4. Locate the gills, these are feathery structures that may be hidden under other organs, there are two of them on each side for a total of four. 5. Follow the gills toward the interior to find an enlarged structure at the base of each gill, this is the gill heart. 6. All the way toward the fin is a whitish or yellowish structure, this is the reproductive gonad. The male gonad is generally white; the female gonad is usually more yellow to clear & may contain eggs. 7. Find the hard point at the end of the fin and gently grip it with forceps pulling away from the squid. In this way you should be able to remove in one piece the pen. The pen is the only remains of an ancient shell found in cephalopod ancestors. The pen supports the mantle & is a site for muscle attachment. 8. Find the ink sac; this is a small dark, silvery sac near the siphon. BE CAREFUL AS THE INK WILL STAIN YOUR CLOTHING! 9. (Optional) if you can extract the pen, you may carefully dip it into the ink sac & write your initials in the box below, be careful because the ink will stain! 4 Use the diagram below to assist you in locating the following organs in your squid: ink sac, fins, gonad, gills, mantle, arms, tentacles, siphon, gill heart A. cecum B. fins C. gonad D. vena cava E. gill heart F. gills G. ink sac H. siphon I. tentacles J. arms K. eye L. intestines M. penis (male only) N. mantle 5 Questions 1. Is your squid a male or female? How can you tell? _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 2. How many gills does the squid have? _________________________________________________________________ 3. How many hearts do squid have? __________________________________________ 4. What is the difference between these hearts? _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 5. Squids have evolved and no longer have a true shell. What structure is the only remains of their shell & what is its purpose? _________________________________________________________________ _________________________________________________________________ 6