Download Table S4. Primers designed for the genes of interest selected for

Table S4. Primers designed for the genes of interest selected for cloning and qRT-PCRa
Gene
5’-3’ Primer Sequence
CKS_3263 F: CAGAACTGAATGGCTTTTGC
CDS
R: GGACTTGGTGGTCCACT
CKS_3263
RT
CKS_3793
CDS
F: TATGACCGGAGTTGATTTCTTTGG
R: TGCCCAGGCAGCTAAAATGA
F: ATGCTAGATATCGTCGAACTGTC
R: TCTGTTCATGGTGATAGCGC
CKS_3793 F: CCTTTGTGGGCCTGTTCTTTTT
RT
R: ACCGCCAGATGCTGCACTT
rmf CDS
F: ATGAAGAGACAGAAACGAGACC
R: TCCCAACCAGTGAGACTTAGC
rmf RT
F: CAGAAACGAGACCGCCTTGA
R: GCGTCCTGTAATGCCAGCTT
bfr CDS
F: ATGAAGGGCGATGCGAAAATCATAAG
R: TTACTCTTCTTTGATTTGCGCCTG
bfr RT
F: TGATTACGTAAGCCGCGATATG
R: CAGTCGATATGATGCTCCTCATCTT
CKS_3570 F: TTTATTCACGATCTGATTAACTGGATTGAC
CDS
R: AACACCATACTGACGCTTGAAGC
CKS_3570 F: CTCGATCTCGATACGGTTTCTGA
RT
R: ATCCGTTGCAGGTGCCATT
aceB CDS F: ATGACAGACTCAGTTATTACCCACGAATTACAC
R: TTACGTGCGCTGTCTTTACTTGG
aceB RT
F: TGGCTGGCACATTGTCTCATA
R: GGATCGGCGCGAAGCT
yeaG CDS F: TACCTTGGCACCATTATGTCG
R: ATCGTCGTGTTTCTTCTGCTCATCC
yeaG RT
F: ACCGACCCGAAAGCGAAAT
R: CGTCCACGCCCGCATA
CKS_2505 F: CTACTGGCTTCCGTATTCCATCG
CDS
R: ATGAACGGCTTAATGAAACTCGATCG
Function
(Annealing
Temperature
°C)
Cloning
CKS_3263
coding region
qRT-PCR
(60°C)
Cloning
CKS_3793
coding region
(Kernell
Burke et al.,
2015)
qRT-PCR
(64°C)
(Kernell
Burke et al.,
2015)
Cloning rmf
coding region
qRT-PCR
(64°C)
Cloning bfr
coding region
qRT-PCR
(60°C)
Cloning
CKS_3570
coding region
qRT-PCR
(64°C)
Cloning aceB
coding region
qRT-PCR
(60°C)
Cloning yeaG
coding region
qRT-PCR
(64°C)
Cloning
CKS_2505
coding region
CKS_2505 F: TTTGGGCATCGAGCATCTTC
RT
R: CGCTTTATCACCCGCAGTATTG
hupA CDS F: AAAGCTGACCTGTCTAAAACCCAG
R: ACGGCGTCTTTCAGAGCTTTACC
hupA RT
F: GCTGAGCGTACCGGTCGTAA
R: TGCCGCAGCGATTTTGAT
CKS_4537 F: CGGAAGTTCTGAATAATGGCTGCG
CDS
R: CTGATACAAACCCAAGCCCCACG
qRT-PCR
(64°C)
Cloning hupA
coding region
qRT-PCR
(64°C)
Cloning
CKS_4537
coding region
qRT-PCR
(60°C)
Cloning recF
coding region
qRT-PCR
(60°C)
Cloning atpD
coding region
qRT-PCR
(64°C)
Cloning gyrB
coding region
CKS_4537 F: CAAGAGCCTTTTGGGCATCCT
RT
R: CTCGGTCGCATTCGAAACC
recF CDS F: TTAATCTTTAGGTTGAACCGCTATTTTACCCTG
R: GCTTTAACCCGCCTGCTAATAAAAGATTTTCG
recF RT
F: AGAGGCAAAGTCATCAATGAGGTAAA
R: GGGCGAGTTCCTGACTAACCA
atpD CDS F: TTAAGAATGGTGATGCTCGTCTGG
R: CTTCTTCGATGGCACCAACC
atpD RT
F: GGTGCGGGTGTGGGTAAAA
R: GCTCAGCCGCAATGTTACG
gyrB CDS F: TTAGATATCGATGTTAGCGGCTTTCAGC
R:
ATGTCGAATTCTTATGACTCTTCAAGTATCAAAGTTCTG
gyrB RT
F: GACGTGACCACGCTCAATAATTTC
qRT-PCR
R: CGGCTCGCACATTCGTACA
(60°C)
a
Primers listed as coding DNA sequence (CDS) were for the cloning of each gene into pGEM-T,
and RT was for the qRT-PCR protocol.