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Table S4. Primers designed for the genes of interest selected for cloning and qRT-PCRa Gene 5’-3’ Primer Sequence CKS_3263 F: CAGAACTGAATGGCTTTTGC CDS R: GGACTTGGTGGTCCACT CKS_3263 RT CKS_3793 CDS F: TATGACCGGAGTTGATTTCTTTGG R: TGCCCAGGCAGCTAAAATGA F: ATGCTAGATATCGTCGAACTGTC R: TCTGTTCATGGTGATAGCGC CKS_3793 F: CCTTTGTGGGCCTGTTCTTTTT RT R: ACCGCCAGATGCTGCACTT rmf CDS F: ATGAAGAGACAGAAACGAGACC R: TCCCAACCAGTGAGACTTAGC rmf RT F: CAGAAACGAGACCGCCTTGA R: GCGTCCTGTAATGCCAGCTT bfr CDS F: ATGAAGGGCGATGCGAAAATCATAAG R: TTACTCTTCTTTGATTTGCGCCTG bfr RT F: TGATTACGTAAGCCGCGATATG R: CAGTCGATATGATGCTCCTCATCTT CKS_3570 F: TTTATTCACGATCTGATTAACTGGATTGAC CDS R: AACACCATACTGACGCTTGAAGC CKS_3570 F: CTCGATCTCGATACGGTTTCTGA RT R: ATCCGTTGCAGGTGCCATT aceB CDS F: ATGACAGACTCAGTTATTACCCACGAATTACAC R: TTACGTGCGCTGTCTTTACTTGG aceB RT F: TGGCTGGCACATTGTCTCATA R: GGATCGGCGCGAAGCT yeaG CDS F: TACCTTGGCACCATTATGTCG R: ATCGTCGTGTTTCTTCTGCTCATCC yeaG RT F: ACCGACCCGAAAGCGAAAT R: CGTCCACGCCCGCATA CKS_2505 F: CTACTGGCTTCCGTATTCCATCG CDS R: ATGAACGGCTTAATGAAACTCGATCG Function (Annealing Temperature °C) Cloning CKS_3263 coding region qRT-PCR (60°C) Cloning CKS_3793 coding region (Kernell Burke et al., 2015) qRT-PCR (64°C) (Kernell Burke et al., 2015) Cloning rmf coding region qRT-PCR (64°C) Cloning bfr coding region qRT-PCR (60°C) Cloning CKS_3570 coding region qRT-PCR (64°C) Cloning aceB coding region qRT-PCR (60°C) Cloning yeaG coding region qRT-PCR (64°C) Cloning CKS_2505 coding region CKS_2505 F: TTTGGGCATCGAGCATCTTC RT R: CGCTTTATCACCCGCAGTATTG hupA CDS F: AAAGCTGACCTGTCTAAAACCCAG R: ACGGCGTCTTTCAGAGCTTTACC hupA RT F: GCTGAGCGTACCGGTCGTAA R: TGCCGCAGCGATTTTGAT CKS_4537 F: CGGAAGTTCTGAATAATGGCTGCG CDS R: CTGATACAAACCCAAGCCCCACG qRT-PCR (64°C) Cloning hupA coding region qRT-PCR (64°C) Cloning CKS_4537 coding region qRT-PCR (60°C) Cloning recF coding region qRT-PCR (60°C) Cloning atpD coding region qRT-PCR (64°C) Cloning gyrB coding region CKS_4537 F: CAAGAGCCTTTTGGGCATCCT RT R: CTCGGTCGCATTCGAAACC recF CDS F: TTAATCTTTAGGTTGAACCGCTATTTTACCCTG R: GCTTTAACCCGCCTGCTAATAAAAGATTTTCG recF RT F: AGAGGCAAAGTCATCAATGAGGTAAA R: GGGCGAGTTCCTGACTAACCA atpD CDS F: TTAAGAATGGTGATGCTCGTCTGG R: CTTCTTCGATGGCACCAACC atpD RT F: GGTGCGGGTGTGGGTAAAA R: GCTCAGCCGCAATGTTACG gyrB CDS F: TTAGATATCGATGTTAGCGGCTTTCAGC R: ATGTCGAATTCTTATGACTCTTCAAGTATCAAAGTTCTG gyrB RT F: GACGTGACCACGCTCAATAATTTC qRT-PCR R: CGGCTCGCACATTCGTACA (60°C) a Primers listed as coding DNA sequence (CDS) were for the cloning of each gene into pGEM-T, and RT was for the qRT-PCR protocol.