Download Mouse/Human Integration and future visions

Mouse/Human Integration and
future visions
All Hands Meeting 2005
Mouse/Human Integration and future visions
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Facilitators/ Leaders : Mark Ellisman
and Anders Dale
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Morphometry BIRN
Anders Dale
• Silvester Czanner
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Function BIRN
• Michelle Diaz
• Martina Panzenboeck
• Steve Potkin
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Mouse BIRN
• Rob Williams,
• Van Phung
Diana Price
• Alexanda Badea
Jyl Boline
Al Johnson
Anjum Sharief
Mark Ellisman
Dave Shattuck
Representing Ontology Working Group
Bill Bug
Working group goals
 1- catalyze connections between specific researchers in
the mouse and human testbeds
 2- instigate focused work on informatics /ontology
frameworks that will help link the model
descriptions/database to the human degenerative
disorder database, clinical progression/ genetics etc.
 3- set clear 6 month and 1 year goals that are partnered
between testbeds - where possible.
Intended deliverables for this session
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Develop a cross-species anatomical and molecular
onotology/homology map
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Determine possible parallels in mouse/human
studies - considering options above.
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Determine possible partnerships - intra-BIRN and
outside BIRN.
Identified activities related to mouse-human
integration efforts
 Genetics and gene analyses
 MRI Imaging
• In vivo imaging (extension of imaging strategies to clinical)
 Mouse-human context of models
 T1-T2
• Contrast mechanisms
• Unwarping and calibration
• Automated segmentation strategies (template driven –
voxel x voxel – adapted to mouse)
 Facilitated by higher resolution scans (increased resolution and
contrast)
 Link to high resolution light and electron microscopy to drive
segmentation through knowledge
 Additional segmentation strategy (surface-based)
 Same structure/context of mouse models – eg. alpha-synuclein
Deliverables: AD/MCI (Mouse-MBIRN)
 Strategy for Alzheimer’s-related animal model: APP
tet-off system tg animals
• Establish contact with J. Jankowsky
• Animal acquisition
 Scanning of AD/MCI human tissues for MRI
• ADRC (Patient and control tissues)– Contact: E. Masliah
• Materials for scanning at CIVM
• Need to identify regions: eg. Entorhinal cortex, ventral
hippocampus
• Tissue request: http://www.neurosci.ucsd.edu/request_materials.asp
Deliverables: Parkinson’s disease (Mouse-FBIRN)
 Micro-PET for alpha-synuclein tg animals
• Steve Potkin & Anders Dale
• D1/D2 probes
• Experimental details TBD: Protocols, transfers, group
design
Deliverables: Schizophrenia (FBIRN-Mouse)
 Links in Schizophrenia: neuroanatomical and
behavioral correlates
• Cross species model (rodent-human) of sensorimotor
gating deficits: Prepulse Inhibition (PPI)
• Action Items:
 Do animals for inbred strains GAJ is scanning have differences in
PPI
 Anders and Anjum – identify a list of structures of interest within the
known PPI circuitry
 Consult Potkin for specifics to proceed
 Later: auditory oddball task in mice
Deliverables: Image analysis tools (ALL)
 Many codes developed for work in human imaging,
analyses and visualization now being adjusted to
accommodate imaging data from animal imaging
(MRI, LM, EM)
• LDDM
• Skull stripping
• Segmentation
Ontological engineering and data integration: Goal
compatible “knowledge maps” (ALL)
 Mouse to human – anatomical mapping
• Anatomy at appropriate granularity
• Disease
• Use same tool: eg. Free Surfer-like
 Exporting ontological terms & plans exported to tool like Smart
Atlas
 Disease maps
 Pick a dataset –related to particular disorder
• Parkinson’s
• MAD
 Use cases: questions to be asked