Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Bell Ringer • The arrow in the figure is pointing to what? • A proteins __________ structure is seen below. Objective • Use appropriate microbiology media, test systems, and lab equipment. • By the end of the lesson students will – Perform an aseptic transfer of sterile broth media from one test tube to another – Obtain pure colonies by performing a T-streak – Complete pre-lab questions on the handout • 5 points for no contamination • 5 points for pure colony • 12 points Worksheet Transfer of Bacteria: Aseptic Technique Background • Inoculate: bacteria are introduced into culture media – This must be accomplished without contamination, or introducing unwanted mos into the media • Aseptic technique: To transfer microbes from one place to another without contamination of the culture, the sterile medium, or the surroundings Background • All culture media is sterilized or rendered free of all living organisms before use – Therefore, containers of media, test tubes, and Petri dishes should not be opened until ready for use Types of Media • Broth cultures: liquid media used to provide large quantities of bacteria room to grow – Fast growing conditions, easy to transport • Agar slants: test tubes containing solid culture media that were left at an angle when solidifying – Provide a solid growth surface – Easy to transport and store – What is used to solidify media? Types of Media • Agar Plates (petri dishes): Solid culture media used to determine the colony morphology of a organism – Create pure cultures Aseptic Technique • Inoculating loop – Transferring instrument of cultured bacteria – Sterilize before and after use – Made of metal Bacterial Cultures • Pure culture – a culture with a single species of cells • Colony – a cluster of cells originating form the multiplication of a single cell • Subculture – the transfer of a microorganism from the original culture to a new one Streak Plate for Isolation • Spreading of organisms on a plate such that individual cells become separated and grow into individual colonies • This method can yield a pure colony – One specie • Isolation is commonly performed when you want to identify an organism – An individual species can be separated out from the mixed culture Streak Plate for Isolation Bacteria is streaked across the surface of the medium During streaking, cell density decreases Individual cells will be deposited on the agar These individual cells will grow into colonies or colony forming units (CFU) Streak Plate for Isolation • T-streak method – Flame loop in-between – Incubate plates upside-down… Why? Label All Tubes and/or Plates • Name • Date • Sample – Bacteria that is in the tube/plate Practice • Complete Worksheet