Download Midterm Lab Practical Study Guide 2015

MCC BIO 205 Study Guide Lab Practical
Note: Any pre-assessment question may be asked on the lab practical.
Ex. 1 Safety
1. Know the 20 lab safety items listed in manual
2. State where the following items are disposed: used glass test tubes, used glass
slides, broken glass, used latex gloves, paper towels used in bench disinfection,
uncontaminated trash, contaminated Petri plates, Qtip swabs use to clean
microscopes
Ex. 2 Microscope
1. Identify the parts of a bright field microscope
2. State the function of each part of the microscope
3. Calculate the total magnification of an object
4. Explain how to clean the microscope properly
5. Explain the purpose of the immersion oil when using the 100X objective
Ex. 7 Aseptic Transfer and Isolation Techniques
1. Define ubiquitous, pure culture, colony, turbidity, media, inoculum, and aseptic
technique
2. Explain the importance of
a. Allowing the loop to cool before obtaining your bacterial sample
b. Holding the test tube cap with your little finger while taking your bacterial
sample
c. Obtaining a pinpoint amount of sample as your inoculum
3. Demonstrate the correct procedure to streak for isolation
4. Explain why the agar plates are labeled on the bottom and inverted when incubated
5. State the criteria used to describe a bacterial colony
Ex. 8 Gram Stain
1. List, in order, the reagents used in the traditional gram stain procedure
2. State the function of each reagent
3. Explain the mechanism of the gram stain (why gram negative cells lose the
primary dye during decolorization)
4. Correctly interpret and report the gram reaction and morphology of gram
stained bacteria
5. Explain the difference between a simple stain and a differential stain
Ex. 9 Acid-Fast Stain
1. State the two genera that are identified using the acid-fast stain. State the
diseases caused by acid-fast bacteria.
2. Distinguish acid-fast organisms from non-acid-fast organisms under the
microscope
3. Explain why certain organisms are “acid-fast”
4. Differentiate between the decolorizer used in the acid-fast stain and the
Gram stain
5. State, in order, the three reagents used in the acid-fast stain
Ex. 11 Endospore Stain
1. Name two medically important genera of bacteria that produce endospores
2. Differentiate the vegetative cell from the spore in a Schaffer-Fulton stain
3. Describe the process used to stain the relatively impermeable endospores
Ex. 12 Negative Stain
1. Differentiate between a negative stain and a positive stain
2. Identify a bacterial capsule in a negative stain
3. Describe the appearance of the bacteria and background of a negative stained
bacterial smear
4. State two advantages of a negative stain over a positive stain
Effects of Oxygen
1. Know the different classification of organisms based on oxygen requirements.
2. Know the different enzymes that remove toxic oxygen.
3. Know the different growth conditions (ie gas pak, candle jar, etc.) used to isolate
different organisms based on oxygen requirements.
4. Know how the organisms used in lab exercise were classified.
Ex. 13 Transformation
1. Describe the genes located on the pGLO plasmid
2. Describe the protein encoded in the pGLO genes
3. Define “competent” and describe two methods used to make the E. coli competent
4. Explain the regulation mechanism of the Green Fluorescent Protein gene expression
Ex. 14-Antibiotic Sensitivity Test and Disinfectant Effectiveness
1.
2.
3.
4.
Compare the Kirby-Bauer test with the Minimal Inhibitory Concentration test
State the principle of the beta-lactamase test and interpret test results
State the media used for the antibiotic sensitivity test
Given an antibiotic sensitivity chart and Kirby-Bauer test, measure the zone sizes
of inhibition in millimeters and interpret the results