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The Microscope
Exercise A: Compound Light Microscope
Exercise A: Compound Light Microscope
• Our microscopes have 3-4
objectives
• Each objective has the
magnification power engraved on
the side:
• 3.5x or 4.5: scanning
objective
• 10x: low power objective
• 40x: high power objective
• 100x: oil immersion objective
Take a slide from your box and test the magnification for each
objective. You should have oil immersion in your table
Exercise A: Compound Light Microscope
• To focus use first the:
• Coarse-adjustment knob
using the low power objective
only!
• Then switch objective and focus
using the fine-adjustment
knob
• These microscopes are
parafocal which means that the
image stays in focus from one
magnification power to the
other.
Take a slide from your box and test the magnification for each
objective. You should have oil immersion in your table
Exercise A: Compound Light Microscope
WHAT IS THE MAGNIFICATION OF YOUR OBJECTIVES?
If your ocular is 10x and the objective is 3.5X the
magnification is: 10 x 3.5=35
• Calculate the magnification for:
• Scanning lens
• Low-power lens
• High-power lens
• Oil-immersion lens
Exercise A: Compound Light Microscope
• Find the diaphragm: regulates the
light
Using the same slide open and
close the diaphragm to regulate
the light.
Exercise A: Compound Light Microscope
ALWAYS take care of your
assigned microscope by:
• Grab it by the arm.
• Cleaning the lenses using the
cleaning lens paper. Never use
alcohol!
• When done, put the
microscope in ‘resting
position’.
YOU ARE RESPONSIBLE FOR YOUR MICROSCOPE ANY THING THAT
HAPPENS YOU NEED TO REPORTED. IF BREAKS IN YOUR WATCH YOU
PAID FOR IT $800!
Exercise B: Dissecting Microscope
There are several dissecting
microscopes in your table
• This scope is to see larger
things e.g. scale of a fish, the
skin of a shark, the eye of an
insect.