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Lecture PBPM - Primary plant cell wall Primary plant cell wall 1/21 Lecture PBPM - Primary plant cell wall: topics Topics diversity of the plant cell wall cell wall structure cell wall sugars molecular model of the cell wall orientation of microfibrils cellulose synthesis antibody techniques in cell wall analysis cell growth and the cell wall acid growth of the cell wall and expansins en matic cell wall enzymatic all degradation 2/21 Lecture PBPM - Diversity of the plant cell wall: cross section of clover (Trifolium) epidermis cortex phloem hl fibers fib phloem cambium xylem pith 3/21 Lecture PBPM - Plant cell wall structure in the electron microscope (Trifolium) surface view: fibrils cross section: layers cuticle outer wall layers inner wall layers 4/21 Lecture PBPM - Structural components of plant cell walls starch: 1,4-α-D-glucose helical structure 5/21 Lecture PBPM - Common sugars of plant cell walls 6/21 Lecture PBPM - Schematic diagram of the plant cell wall 7/21 Lecture PBPM - Structural model of the plant cell wall: cellulose structure 8/21 Lecture PBPM - Structural model of the plant cell wall: cellulose linkage 9/21 Lecture PBPM - Orientation of microfibrils and the direction of cell growth 10/21 Lecture PBPM - Orientation of cellulose microfibrils parallel orientation i t ti diffuse orientation 11/21 Lecture PBPM - Cellulose synthesis 30 nm 0.1 µm 12/21 Lecture PBPM - Cellulose synthesis 13/21 Lecture PBPM - Antibodies for in situ cell wall analysis cell wall stained in blue by calcofluor two monoclonal antibodies against different epitopes of pectic homogalcturonan 14/21 Lecture PBPM - Structure of a sclereid from Podocarpus 15/21 Lecture PBPM - Tip growth vs. diffuse growth of plant cells 16/21 Lecture PBPM - Acid growth of plant cell walls 17/21 Lecture PBPM - Role of proteins (expansins) in acid growth of plant cell walls 18/21 Lecture PBPM - Mechanism of expansin function in plant cell walls 19/21 Lecture PBPM - Enzymatic cell wall degradation in plant/pathogen interaction 20/21