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Lecture PBPM - Primary plant cell wall
Primary plant cell wall
1/21
Lecture PBPM - Primary plant cell wall: topics
Topics
diversity of the plant cell wall
cell wall structure
cell wall sugars
molecular model of the cell wall
orientation of microfibrils
cellulose synthesis
antibody techniques in cell wall analysis
cell growth and the cell wall
acid growth of the cell wall and expansins
en matic cell wall
enzymatic
all degradation
2/21
Lecture PBPM - Diversity of the plant cell wall: cross section of clover (Trifolium)
epidermis
cortex
phloem
hl
fibers
fib
phloem
cambium
xylem
pith
3/21
Lecture PBPM - Plant cell wall structure in the electron microscope (Trifolium)
surface view: fibrils
cross section: layers
cuticle
outer wall
layers
inner wall
layers
4/21
Lecture PBPM - Structural components of plant cell walls
starch:
1,4-α-D-glucose
helical structure
5/21
Lecture PBPM - Common sugars of plant cell walls
6/21
Lecture PBPM - Schematic diagram of the plant cell wall
7/21
Lecture PBPM - Structural model of the plant cell wall: cellulose structure
8/21
Lecture PBPM - Structural model of the plant cell wall: cellulose linkage
9/21
Lecture PBPM - Orientation of microfibrils and the direction of cell growth
10/21
Lecture PBPM - Orientation of cellulose microfibrils
parallel
orientation
i t ti
diffuse
orientation
11/21
Lecture PBPM - Cellulose synthesis
30 nm
0.1 µm
12/21
Lecture PBPM - Cellulose synthesis
13/21
Lecture PBPM - Antibodies for in situ cell wall analysis
cell wall stained
in blue by calcofluor
two monoclonal antibodies
against different epitopes of
pectic homogalcturonan
14/21
Lecture PBPM - Structure of a sclereid from Podocarpus
15/21
Lecture PBPM - Tip growth vs. diffuse growth of plant cells
16/21
Lecture PBPM - Acid growth of plant cell walls
17/21
Lecture PBPM - Role of proteins (expansins) in acid growth of plant cell walls
18/21
Lecture PBPM - Mechanism of expansin function in plant cell walls
19/21
Lecture PBPM - Enzymatic cell wall degradation in plant/pathogen interaction
20/21
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