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Industriell bruk og nytte av enzymer by Kjartan Sandnes, Dr. Daglig leder og forskningsleder Biorefining salmon co-products Enzymatic hydrolysis Oil Water and enzymes Water soluble protein Exact time and temp Sediment Some key figures • Norwegian sea areas 7 times the size of land area • All included, Norway is the 13th largest country in the world • Second largest seafood export nation • 53 bill. NOK • 33 mill seafood meals on the table every day Norway 2012: «Non-edible fish raw materials» White fish (cod, haddock, saithe) Herring Aquaculture (salmon, rainbow trout) Total co-products 325.000 239.000 350.000 914.000 «Industrial fish» (to the fish meal- and oil industry 2012) T O T A L (not for direct human consumption) 202.000 1.116.000 NOTE! Seaweeds and shellfish not included Source: fhf, Sildesalgslaget Source: fhf, Sintef, Sildesalgslaget Biomega AS – a blue biorefinery • • • • • • 2000: Marine Bioproducts AS established 2002: Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required 2007: New site acquired for new factory 2011: Six patent families filed so far 2011: 30.000 tons raw materials processed . Turnover 107 mill NOK 2012: New factory with a capacity of 60.000 tons annually (130 mill NOK inv.) - opened May 2012 6 Raw material transportation to Biomega – heads and backbones Constant efforts to improve quality (cooling systems) and logistics Raw material transportation to Biomega – viscera in bulk by truck Page 8 New processing plant production from May 2012 9 Overview of new plant 10 Biorefining - current and next generation products MB Process Hydrolysate Oil Sediment Bones (soluble protein) (fat) ((unsoluble protein) (minerals, protein) NEW AND INNOVATIVE PROCESSES enzyme technology – extraction – separation - isolation BIOACTIVE PEPTIDES PEPTONES FLAVOURS MODIFIED LIPIDS PHOSPHOSLIPIDS POWDERS EXTRACTS E X A M P L E S MARINE MINERALS GLUCOSAMINES CHONDROITIN BIOACTIVE PEPTIDES How enzymes can be used Herring Hydrolysates Salmon Hydrolysates HH 1 HH 2 MB HR 1 MB HR 2 MB HR 3 0 0 2.5 0.2 0.0 30 000 – 20 000 daltons 1.3 0 0.7 0.2 0.0 20 000 – 10 000 daltons 1.2 0.2 12.6 7.1 4.6 10 000 – 5 000 daltons 12.4 5.2 17.8 15.5 10.0 5 000 - 1 000 daltons 14.6 10 13.3 9.7 10.2 1 000 - 53.1 66.1 38.3 40.5 33.9 17.4 18.4 14.7 26.7 41.3 2100 1000 4900 2800 1900 Test sample Peptide size distribution (% of total detected) > 30 000 daltons 100 daltons < 100 daltons Rough estimated average Mw Carbon source Nitrogen source Micronutrients Glucose, Glycerol Ammonium salts, Nitrates, Peptone (amino acids/peptides) Phosphates, Sulphates, Trace metals, Vitamins SEM Typical growth medium Fungi Yeasts Antibiotics Bacteria Enzymes Vaccines Mammalian cells Other Saccharomyces cerevisiae Growth Curves 6 LACH SOYP OXYE 5 CASA GELP SEM OD600 4 BDYE MBPT “good for yeasts” from supplier 2 3 2 highest AN from supplier 1 1 highest AN from supplier 2 0 0 1 2 3 4 5 6 7 8 9 10 Time (h) 11 12 13 14 15 16 17 18 19 20 Mask writer Thank you!